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Plasmid#129681PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationE848A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pHBDuet139
Plasmid#121913PurposeCharge-Introduced NpuDnaB mini-inteinDepositorInsertCI-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet140
Plasmid#121914PurposeOrthogonal NpuDnaB mini-intein inteinDepositorInsertOth-NpuDnaBΔ290 intein
UseTagsGB1 and His6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)
Plasmid#132637Purposeexpresses mouse Tankyrase1 ARC2-3 (308-655) in E. coliDepositorInsertTankyrase1 (308-655)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-amidase
Plasmid#128135PurposeThe cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertamidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(∆77-91)
Plasmid#121516PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationdeleted amino acids 77-91PromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
mdm1_pJet1.2
Plasmid#121977Purposefor generating mdm1 riboprobes for WISH in zebrafishDepositorInsertmdm1 (mdm1 Zebrafish)
UseCloning vectorTagsExpressionMutationPromoterT7Available sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 uvrA
Plasmid#112892PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertuvrA-like protein
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOC5570372 NPFF receptor
Plasmid#115378PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5570372 NPFF receptor (LOC5570372 Aedes aegypti)
UseTagsExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOC5567066 Gastrin-releasing peptide receptor
Plasmid#115372PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOC5570884 Cardioacceleratory peptide receptor
Plasmid#115373PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5570884 Cardioacceleratory peptide receptor (LOC5570884 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOC5570885 Cardioacceleratory peptide receptor
Plasmid#115375PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5570885 Cardioacceleratory peptide receptor (LOC5570885 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LOC5574680 Atrial natriuretic peptide receptor
Plasmid#115374PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertLOC5574680 Atrial natriuretic peptide receptor (LOC5574680 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Cul1a-3'UTR
Plasmid#97152PurposeFor dual luciferase report assayDepositorInsertCul1a 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Cul1b-3'UTR
Plasmid#97153PurposeFor dual luciferase report assayDepositorInsertCul1b 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-Traf6-3'UTR
Plasmid#97154PurposeFor dual luciferase report assayDepositorInsertTraf6 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertUB2" (SNRPB2 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only