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Plasmid#170577PurposeGateway destination clone of CAMKK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CAMKK2 (CAMKK2 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-ccdB-Firefly
Plasmid#87074PurposeLentiviral Gateway-compatible expression vector backbone with a C-terminal Firefly luciferaseDepositorTypeEmpty backboneUseLentiviralTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRD3 gRNA (BRDN0001147217)
Plasmid#76094Purpose3rd generation lentiviral gRNA plasmid targeting human BRD3DepositorInsertBRD3 (BRD3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOE-E2-T2A-mCherry
Plasmid#190807PurposeExpress APOE E2 allele from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterUbi CAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1355
Plasmid#143100PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertILF3 (ILF3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK 3xFLAG-TWIST2 neo
Plasmid#197936PurposeLentiviral expression vector for an inducible 3xFLAG-tagged TWIST2DepositorInsertTWIST2 (TWIST2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2-BSD
Plasmid#191101PurposeLentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD markerDepositorInsertNRCH-PE2-BSD
UseCRISPR and LentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP
Plasmid#194897PurposeDox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.DepositorInsertsdLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble
UseLentiviralTagsHAExpressionMammalianMutationHuman codon optimized catalytically inactive LbCp…PromoterTRE and U6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
BRD3 gRNA (BRDN0001146442)
Plasmid#76092Purpose3rd generation lentiviral gRNA plasmid targeting human BRD3DepositorInsertBRD3 (BRD3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-Floxed Luc
Plasmid#60622PurposeExpresses a conditional luciferase cassette dependent on Cre mediated recombinationDepositorInsertFloxed Luciferase
UseCre/Lox, Lentiviral, and LuciferaseTagsExpressionMutationPromoterhuman UbCAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro
Plasmid#170467PurposeThe plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistanceDepositorArticleInsertNucleocapsid
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterEF1Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-puro-2A-BLM
Plasmid#127641PurposeOver-expression of human BLMDepositorInsertBLM (BLM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgMark2-mU6-sgMark3-hU6-sgMark4
Plasmid#177235PurposeExpresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinaseDepositorInsertsgMark2/sgMark3/sgMark4
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2
Plasmid#177231PurposeExpresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgNeo2
UseLentiviralTagsExpressionMutationPromoterbU6/mU6/hU6Available sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0489
Plasmid#143029PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFP91 (ZFP91 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0794
Plasmid#142239PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertATF7 (ATF7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2718
Plasmid#142131PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertLRPPRC (LRPPRC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3165
Plasmid#144641PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTRIM28 (TRIM28 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-KLF4
Plasmid#130694PurposeLentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.DepositorInserthuman KLF4 (KLF4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only