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Showing: 11241 - 11280 of 156661 results

  3. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  16. pCEV-G2-Km ymNeongreen

    Plasmid
    #193959
    Purpose
    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast
    Depositor
    Bas Teusink
    Insert
    ymNeonGreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193959-plasmid-map-sequence-id-383894

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  20. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Tags
    PAmCherry1
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200419-plasmid-map-sequence-id-405382

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  22. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  27. TFORF0239

    Plasmid
    #142385
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYCL (MYCL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142385-plasmid-map-sequence-id-391445

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  30. p5E-mpeg1.1

    Plasmid
    #75023
    Purpose
    Can be used to drive expression in zebrafish microglia.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Tags
    Expression
    Mutation
    Promoter
    mpeg1.1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75023-plasmid-map-sequence-id-141914

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  39. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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Showing: 11241 - 11280 of 156661 results