-
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mWasabi-G
Plasmid#164161PurposeExpresses mWasabi under the control of CAG promoter, basic vector, multiclonig site at C terminalDepositorInsertmWasabi
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR8-YFP
Plasmid#13024DepositorInsertToll-Like Receptor 8 (TLR8 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMVTNT-T7-KPNA2
Plasmid#26678DepositorInsertKaryopherin Alpha 2 (KPNA2 Human)
UseTagsT7 (MASMTGGQQMG)ExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHD422N
Plasmid#194432PurposeAPOBEC1 fused to YTHD422NDepositorInsertAPOBEC1-YTHD422N (APOBEC1 Human)
UseCRISPRTagsAPOBEC1-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…PromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAW005-2
Plasmid#85611PurposeEmpty gRNA delivery vector for Bacillus subtilisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom
Plasmid#135630PurposeAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertdTom-nlsdTom
UseAAVTagsExpressionMutationNonePromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsUseTagsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_479
Plasmid#179099PurposeBE Cas expressionDepositorInsertABE8e (SpG)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF112
Plasmid#135920PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseLuciferase; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF.PGK.GFP
Plasmid#17618DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA E545K
Plasmid#12525DepositorInsertPIK3CA E545K (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationE545K. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG104 FAST
Plasmid#130720PurposeExpresses FAST (also called YFAST) in mammalian cellsDepositorInsertFAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NEmut
Plasmid#123310PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neuronsDepositorHas ServiceAAV Retrograde and AAV9InsertGPCR activation based NE control sensor GRAB_NEmut
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_SNF2L-opt_p48
Plasmid#102243PurposeExpression of human codon optimized human SNF2L and pRbAp48 from pFastBac dualDepositorUseTagsExpressionInsectMutationcodon optimized for expressionPromoterPolyhedrin and p10Available sinceNov. 1, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZsGreen1-cMYC/pLVX-Puromycin
Plasmid#180278PurposePlasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).DepositorInsertZsGreen1-cMYC fusion protein MYC (MYC Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TurboID-EV
Plasmid#215085PurposeExpresses TurboID in mammalian cells from a retroviral vector.DepositorInsertTurboID (birA )
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only