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Plasmid#120442PurposeBarcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertGATA1 (GATA1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR
Plasmid#118155PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterhPGK and U6Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn LAMP1-msGFP (JV012)
Plasmid#179728PurposeLentiviral expression of LAMP1-msGFP for use as a lysosomal markerDepositorInsertLAMP1 (Lamp1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgBAP1-2
Plasmid#125838PurposeKO BAP1 geneDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro
Plasmid#180268PurposeLentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNADepositorInsertGARP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-mNeonGreen
Plasmid#167587PurposeExpresses human CPSF6 fused to mNeonGreenDepositorInsertCPSF6-mNeonGreen (CPSF6 Human)
UseLentiviralTagsmNeonGreenExpressionMutationWTPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUbC-GBX2-CDX124-Cas9-T2A-GFP
Plasmid#192287PurposeExpresses Cas9-T2A-GFP and four sgRNAs: GBX2, CDX1, CDX2, CDX4DepositorInsertUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-pegRNA-tetra-com vector
Plasmid#136271PurposeExpresses PE2 and Tetra-com modified pegRNA cassetteDepositorInsertPE2 and pegRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF1367
Plasmid#143492PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRREB1 (RREB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT PBRM1- TRIPZ-neo
Plasmid#107406PurposeMammalian expression, inducible PBRM1DepositorInsertPBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorInsertCDK6 (CDK6 Human)
UseLentiviralTagsExpressionMammalianMutationPAM site mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2597
Plasmid#142963PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRORA (RORA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mGSDME F2A
Plasmid#154878PurposeExpresses mGSDME F2A in mammalian cellsDepositorInsertGSDME (Gsdme Mouse)
UseLentiviralTagsExpressionMutationchanges Phenylalanine 2 to AlaninePromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CHEK2 C3.2 gRNA
Plasmid#90628Purpose3rd generation lentiviral gRNA plasmid targeting human CHEK2DepositorInsertCHEK2 (Guide Designation C3.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-EphB2-SV40-GFP (B2G)
Plasmid#65442PurposeExpression of mouse EPHB2 and GFP in mammalian cellsDepositorInsertEPHB2 (Ephb2 Mouse)
UseLentiviralTagsSV40-EGFPExpressionMammalianMutationSee depositor comments below.PromoterUBQAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-mOrange2
Plasmid#110731PurposeMammalian Expression of p14ARF IRES H2B-mOrange2DepositorInsertp14ARF IRES H2B-mOrange2 (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
S1-Fc
Plasmid#164220PurposeExpresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporterDepositorInsertSpike S1 domain (S SARS-CoV)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-eArchT 3.0-EYFP
Plasmid#35513PurposeLentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.DepositorInserteArchT 3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only