Skip to main content
Addgene
Showing: 5601 - 5620 of 13539 results

  5. Desmoplakin II tension sensor (F40-based)

    Plasmid
    #118715
    Purpose
    The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118715-plasmid-map-sequence-id-230444

    Enlarge

  7. Desmoplakin I tension sensor (F40-based)

    Plasmid
    #118725
    Purpose
    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118725-plasmid-map-sequence-id-230441

    Enlarge

  10. Desmoplakin II no force control (F40-based)

    Plasmid
    #118716
    Purpose
    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118716-plasmid-map-sequence-id-230464

    Enlarge

  12. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118726-plasmid-map-sequence-id-230443

    Enlarge

  15. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid
    #176334
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19R, G142D, del156-157, R158G, L452R, T478K, D61…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176334-plasmid-map-sequence-id-347146

    Enlarge

  17. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

    Plasmid
    #184533
    Purpose
    Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-BA.2 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    184533-plasmid-map-sequence-id-361466

    Enlarge

  18. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

    Enlarge

  19. pVRC8400-SARS-CoV-2-RBD-SD1-L452R-T478K-AVI

    Plasmid
    #176456
    Purpose
    Expresses SARS-CoV-2 RBD-SD1 domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD-SD1-L452R-T478K (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    L452R, T478K
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176456-plasmid-map-sequence-id-347373

    Enlarge

  20. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid
    #176325
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.7 (S )
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…
    Promoter
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176325-plasmid-map-sequence-id-347062

    Enlarge

Showing: 5601 - 5620 of 13539 results