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pT2SCb

Plasmid

#59385

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat2

Use

Template

Tags

Expression

Mutation

The 3’-end of the chloramphenicol resistance gene…

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAV123

Plasmid

#63189

Purpose

pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSN838

Plasmid

#184826

Purpose

Expresses human KIF5A(∆exon27) in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

∆exon27 form

Promoter

CMV

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAV124

Plasmid

#63190

Purpose

pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

Plasmid

#207665

Purpose

Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

Depositor

Robert de Vries

Article

Spruit et al bioRxiv 2023.12.15.571923

Insert

Fucosidase E1_10125

Use

Tags

His-tag

Expression

Bacterial

Mutation

amino acids 34-584 were used

Promoter

T7 promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

PL(HrtO)

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163074

Purpose

Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- C11orf83-GFP

Plasmid

#65844

Purpose

Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter AP-1 site mutant pGL3Basic

Plasmid

#32728

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Tags

Expression

Mutation

-947 AP-1 binding site TGAGTCA deletion

Promoter

-962 CCND1 promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-metap2

Plasmid

#100516

Purpose

Expresse Myc tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Flag hMcl-1(T163A)

Plasmid

#25373

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1(T163A) (MCL1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pAAV-syn-IRES-hrGFP

Plasmid

#107549

Purpose

high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only
pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-MBD3_1

Plasmid

#36371

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD3 (MBD3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pAV113

Plasmid

#63180

Purpose

pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pUAST td tomato Rab5 WT

Plasmid

#58446

Purpose

expression of drosophila td tomato Rab5

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Tags

td tomato

Expression

Insect

Mutation

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
FabF (KASII) pet28 N-terminal His Tag

Plasmid

#75017

Purpose

Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

FabF (fabF E. coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_2

Plasmid

#36332

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only