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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

In frame with Cas9

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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PG13-luc (wt p53 binding sites)

Plasmid

#16442

Purpose

Luciferase reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

p53-binding site (TP53 )

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-TVA-IRES-Puro

Plasmid

#172366

Purpose

Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

Depositor

Peter Jonas, Yoav Ben-Simon

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + Puro

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette GFP

Plasmid

#102942

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry-t2a-SOX2-p2a-KLF4

Plasmid

#193296

Purpose

episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Discosoma, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-C1

Plasmid

#54759

Purpose

Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

Plasmid

#111568

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Has Service

AAV5

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-DsRed

Plasmid

#11151

Purpose

Mammalian expression vector for expression of DsRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

DsRed2 from Discosoma sp

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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Lamp1-YFP

Plasmid

#1816

Purpose

Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

lysosome associated membrane protein 1 (Lamp1 Rat)

Use

Tags

YFP

Expression

Mammalian

Mutation

D50E (not important for function of plasmid)

Promoter

Available since

Feb. 16, 2006

Availability

Academic Institutions and Nonprofits only

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CD44S pBabe-puro

Plasmid

#19127

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

CD44 standard (CD44 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Use

Tags

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG mito-GCaMP5G

Plasmid

#105009

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

GCaMP5G

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge