Addgene

Showing: 5581 - 5600 of 22194 results

Results per page:

Sort results by:

pSB_syb25

Plasmid

#197992

Purpose

Expressed sybody 25 as a C-terminal myc-His-tagged construct

Depositor

Simon Newstead

Article

Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

Insert

Sybody 25

Use

Tags

Myc-His

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDXC3MS

Plasmid

#49030

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
N114 nLV EF-1a-Gal4-VP16-PGK-Puro

Plasmid

#44014

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Tags

VP16/Activation/Domain

Expression

Mutation

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-his6-HA3-HALO

Plasmid

#188943

Purpose

T7 expression vector for making his6- and HA3-tagged HALO protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-HALO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UIMLx2

Plasmid

#174888

Purpose

UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

Depositor

Peter Kaiser

Article

Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

Insert

Met4 UIML

Use

Tags

Avi-6xHis-Smt3

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-his6-HA3-CLIP

Plasmid

#188942

Purpose

T7 expression vector for making his6- and HA3-tagged CLIP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-CLIP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Use

Tags

6-His

Expression

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-ZnF-UBPdel

Plasmid

#90299

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Use

Tags

His tag

Expression

Bacterial

Mutation

ZnF-UBP deleted

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ038

Plasmid

#200227

Purpose

universal template to build tRNA-gRNA array for multiplex editing CREEPY

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

tRNA-gRNA scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP NFAT5 (no EGFP)

Plasmid

#13627

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
hygII-UT-ABAleon2.1

Plasmid

#106982

Purpose

ABAleon2.1 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Use

Tags

Expression

Plant

Mutation

Promoter

UBQ10

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyn-AKAR4

Plasmid

#61620

Purpose

Lipid raft-targeted FRET biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

Lyn-AKAR4

Use

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIOM15

Plasmid

#20869

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aadA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
hygII-UT-ABAleon2.15

Plasmid

#106983

Purpose

ABAleon2.15 in plant expression vector (Hygromycin resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Use

Tags

Expression

Plant

Mutation

Promoter

UBQ10

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK138

Plasmid

#73314

Purpose

Pas2p peroxisomal membrane targeting sequence

Depositor

T. J. Kappock

Insert

Pas2p (peroxisomal membrane targeting region)

Use

Tags

His6 and c-myc

Expression

Yeast

Mutation

encodes residues 1-42

Promoter

AOX1

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH341-SUP45(139-437)-GalBD

Plasmid

#29741

Depositor

Tobias von der Haar

Insert

SUP45 (SUP45 Budding Yeast)

Use

Tags

Gal Activation domain and Myc

Expression

Yeast

Mutation

Codons 139-437 of the yeast SUP45 gene only

Promoter

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH342-SUP45(276-437)-GalBD

Plasmid

#29742

Depositor

Tobias von der Haar

Insert

SUP45 (SUP45 Budding Yeast)

Use

Tags

Gal Activation domain and Myc

Expression

Yeast

Mutation

Codons 276-437 of the yeast SUP45 gene only

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS42K_MCP-VP64_C-MS2

Plasmid

#177160

Purpose

Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

MCP-VP64
C-MS2

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-CRM197-4xDQNAT

Plasmid

#128395

Purpose

In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Use

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

T7

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge