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psgRNA-GST-EGFP-GPIBY55

Plasmid

#213720

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Expression

Mutation

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPIDAF

Plasmid

#213719

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Expression

Mutation

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA-GST-EGFP-GPICEAM7

Plasmid

#213721

Purpose

To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

Depositor

Qilai Huang

Article

Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

Insert

EGFP

Use

CRISPR

Tags

GST

Expression

Mutation

Promoter

CMV

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pACBE-ASR (pXK-607)

Plasmid

#208822

Purpose

Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT002-M-HaloTag-1x FLAG

Plasmid

#202493

Purpose

Express "M" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Myristoylation site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT008-ZF6 M-synTF-1x FLAG

Plasmid

#202498

Purpose

Express "M" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Use

Tags

1x FLAG and Myristoylation site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT007-ZF6 synTF-1x FLAG

Plasmid

#202497

Purpose

Express "soluble" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Use

Tags

1x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT006-1x FLAG-HaloTag-PPF

Plasmid

#202496

Purpose

Express "PPF" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Palmitoylation-Palmitoylation-Farnesy…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT010-ZF6 1x FLAG-synTF-G

Plasmid

#202500

Purpose

Express "G" synTF (ZF6)

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

p65-ZF6

Use

Tags

1x FLAG and Geranylgeranyl site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT004-1x FLAG-HaloTag-G

Plasmid

#202495

Purpose

Express "G" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Geranylgeranyl site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoG-NAD-NLS3x

Plasmid

#193822

Purpose

CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD-NLS3x

Use

Tags

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

Plasmid

#193828

Purpose

CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD-NLS3x

Use

Tags

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

Plasmid

#193829

Purpose

CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoL-NAD

Use

Tags

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoB

Plasmid

#193808

Purpose

CMV overexpression of ChemoB in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB

Use

Tags

10xHis

Expression

Mammalian

Mutation

EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoC

Plasmid

#193809

Purpose

CMV overexpression of ChemoC in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC

Use

Tags

10xHis

Expression

Mammalian

Mutation

mCerulean3:T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT-ChemoY

Plasmid

#193810

Purpose

CMV overexpression of ChemoY in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY

Use

Tags

10xHis

Expression

Mammalian

Mutation

Venus:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Use

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82A

Plasmid

#171250

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-M4-AR-W741C-S81A-P82G

Plasmid

#171251

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82G)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Gly mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Gly (AR-W741C-S81A-P82…

Promoter

CMV

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge