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Plasmid#168315PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi514714089
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL80
Plasmid#168314PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi514714081
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL79
Plasmid#168313PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi955708539
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL78
Plasmid#168312PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944239627
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL77
Plasmid#168311PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233294
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL76
Plasmid#168310PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233293
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL75
Plasmid#168309PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233287
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL74
Plasmid#168308PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233281
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL73
Plasmid#168307PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944233277
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL72
Plasmid#168306PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertSi944231404
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY282F
Plasmid#172468Purposeexpresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domainDepositorInsertwbp2nlY282F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa282 to PhenylalaninePromoterSP6Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRTagsExpressionBacterialMutationPromoterPmAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC016.109
Plasmid#171144PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPPC008.106
Plasmid#171141PurposeExpression of J106 scRNA on pBBR1-GmR plasmidDepositorInsertJ106 scRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneExpressionMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlT45A
Plasmid#172464Purposeexpresses protein with one aa substitution to affect potential phosphorylationDepositorInsertwbp2nlT45A
UseXenopus expressionTagsnoneExpressionMutationchanged Threonine at aa45 to AlaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY55F
Plasmid#172466Purposeexpresses protein with one aa substitution to affect potential phosphorylation in ITSM domainDepositorInsertwbp2nlY55F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa55 to PhenylalaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only