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Plasmid#175778PurposePiggyBac Vector expressing GFP-RFP-LGALS3DepositorInsertLGALS3 (LGALS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS3
Plasmid#175756PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3BK51A
Plasmid#175754PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51ADepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationK51APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC150-RFP-EGFP-LGALS3 R186S
Plasmid#175779PurposePiggyBac Vector expressing GFP-RFP-LGALS3 R186SDepositorInsertLGALS3 (LGALS3 Human)
UseTagsExpressionMammalianMutationR186SPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc p31Comet
Plasmid#59833PurposeAllows the integration of myc p31Comet in the genome and Tet-inducible expression.DepositorInsertp31 (MAD2L1BP Human)
UseTagsMycExpressionMammalianMutationPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKH152_PB_flag-LC3B_LDSmut_IRES-BFP
Plasmid#214977PurposeFlag tagged LDS mutant LC3BDepositorInsertFLAG-LC3B_mut (MAP1LC3B Human)
UseTagsExpressionMammalianMutationLDS mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-Mch-MAP1LC3B
Plasmid#201924PurposeFor lentiviral transduction of Mch-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2
Plasmid#175751PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A
Plasmid#175752PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50ADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseLentiviralTagsExpressionMutationY49A L50APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC2_2_dCas9_VP64_sgRNA
Plasmid#163707PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domainDepositorInsertsdCas9
VP64+SV40 NLS
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE
Plasmid#109400PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP proteinDepositorInsertHDR-Enhancer domain of human CtIP protein (RBBP8 Human)
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))
Plasmid#218508Purposeintegrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorInsertmNeongreen
UseTagsmodified ssrA degradation tagExpressionBacterialMutationdeletion of MCS containing Plac_lacZa, introducti…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CtIP
Plasmid#109403PurposeExpresses Cas9 fusion to human CtIP proteinDepositorInsertCtIP (RBBP8 Human)
UseTagsExpressionMutationfull length CtIPPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-APP751
Plasmid#193772PurposeEntry vector for human APP isoform having 751 amino acid residuesDepositorInsertAPP (APP Human)
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-APPSw;T719N
Plasmid#177094PurposeFor lentiviral transduction of APPsw; T719NDepositorInsertAPPsw;T719N (APP Human)
UseLentiviralTagsExpressionMutationT719N, K651N and M652L (please see depositor comm…PromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-Geminin
Plasmid#109401PurposeExpresses Cas9 fusion to Geminin degron domainDepositorInsertGeminin (GMNN Human)
UseTagsExpressionMutationGeminin degron fragmentPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC150-KEIMA-VAPA
Plasmid#212096PurposeFluorescent reporter for VAPA clearance to acidic lysosomesDepositorInsertVAPA (VAPA Human)
UsePiggybacTagsmKeimaExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dK-2xG4S-TMst
Plasmid#145766PurposeExpresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.DepositorInsertkappa-myc-dK-2xG4S-TMst
UseTagsExpressionMammalianMutationThe dK FAP is dL5 with mutations at E50K, L89S, a…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only