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Plasmid#214357PurposeExpresses SpCas9HF1 and puromycin resistance genesDepositorInsertSpCas9HF1 and puromycin resistance genes
UseTagsExpressionMammalianMutationN497A/R661A/Q695A/Q926APromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SlugCas9-P2A-puro
Plasmid#214353PurposeExpresses SlugCas9 and puromycin resistance genesDepositorInsertSlugCas9 and puromycin resistance genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Sa-gRNA-puro
Plasmid#191652PurposeLentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.DepositorInsertS. aureus gRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmceTagsExpressionMutationPromotertetOAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 mKO2 HuR.wt IRES Puro
Plasmid#110387Purposeγ-Retroviral transfer vector for expressing HuR (wild type), V5 and mKO2 tags, IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5 and mKO2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#1
Plasmid#174323PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 S573N
Plasmid#167195Purposepuromycin resistant lentiviral vector for expression of PAK5 S573NDepositorInsertPAK5 S573N
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 T538N
Plasmid#167181Purposepuromycin resistant lentiviral vector for expression of PAK5 T538NDepositorInsertPAK5 T538N
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-3xFLAG-Puro PAK5 V593I
Plasmid#167179Purposepuromycin resistant lentiviral vector for expression of PAK5 V593IDepositorInsertPAK5 V593I
UseLentiviralTagsExpressionMammalianMutationPromoterUBCAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti--Puro-3xFLAG PAK5 S364L
Plasmid#167168Purposepuromycin resistant lentiviral vector for expression of PAK5 S364LDepositorInsertPAK5 S364L
UseLentiviralTags3x flagExpressionMammalianMutationPromoterUBC promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMY-mVenus-P2A-PuroR-HA
Plasmid#163345PurposeRetroviral vector to express cytosolic mVenus and an HA-tagged puromycin selection markerDepositorInsertmVenus
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
UseTagsExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-puro
Plasmid#17923PurposeInsect expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL4.82(hRluc/Puro)
Plasmid#212933PurposeRenilla luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQCXIP-turboRFP
Plasmid#73016PurposeRetroviral expression of turboRFPDepositorInsertTurboRFP
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDA52_Lenti.puro.U6_modified DR
Plasmid#196723PurposeFor cloning pairs of AsCas12a guides with two DRs that are as different as possible.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.84(hRlucCP/Puro)
Plasmid#212935PurposeRapidResponse(TM) Renilla luciferase vector for investigating regions controlling transcriptionDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL4.83(hRlucP/Puro)
Plasmid#212934PurposeRenilla luciferase+PEST vector for investigating faster response to transcriptional changesDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMutationWTPromoterAvailable sinceJan. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits