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pEGFP VAMP7 (1-120)

Plasmid

#42317

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Longin domain (1-120) (VAMP7 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Δ120-220

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
Venus-tBid-pEGFP-C1

Plasmid

#166736

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

tBid (Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Lin28

Plasmid

#92347

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-BRAT1

Plasmid

#161920

Purpose

Expresses human BRAT1 with a N-terminal GFP-FLAG tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

BRCA1-associated ATM activator 1 (BRAT1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-MYO10-HMM

Plasmid

#87256

Purpose

Expression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CAMKIIbeta(T287D)

Plasmid

#21223

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIbeta (Camk2b Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

CAMKIIbeta(T287D) Lab plasmid CF 0001.

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
NLS pEGFP-N3 (1299)

Plasmid

#62043

Purpose

mammalian expression of NLS-EGFP

Depositor

Eric Schirmer

Article

Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

Insert

SV40 NLS

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-MYO7A-TAIL

Plasmid

#89585

Purpose

Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO7A (Myo7a Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation encoding amino acids 870-2166 of MYO7A…

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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NET47 pEGFP-N2 (593)

Plasmid

#61986

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET47 (TM7SF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
METTL7A pEGFP-N2 (890)

Plasmid

#62017

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

METTL7A (METTL7A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
K44A dynamin 2 pEGFP

Plasmid

#34687

Depositor

Sandra Schmid

Insert

dynamin 2 (Dnm2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Lin28

Plasmid

#92346

Purpose

Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-ATXN1-30Q

Plasmid

#33238

Depositor

Huda Zoghbi

Article

Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pEGFP-N2 (1078)

Plasmid

#62028

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20-PAAA

Plasmid

#218581

Purpose

Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

changed Proline 172 to Alanine and Tyrosine 174 t…

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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peGFP C1-SPG20 SC domain

Plasmid

#218580

Purpose

Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

deleted amino acids 1-417

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-HPGD-FLAGC

Plasmid

#161915

Purpose

Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

HPGD (HPGD Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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AnkyrinB-GFP/pEGFP-N1

Plasmid

#197327

Purpose

Plasmid expressing an antigen targeting AnkyrinB

Depositor

Vann Bennett

Insert

AnkyrinB (Ank2 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge