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Plasmid#166929PurposeTrrnB and L3S2P21 terminator part (D)DepositorInsertDOUBLE TERMINATOR ( TrrnB - L3S2P21) _BLOCK D - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD010
Plasmid#116851PurposeExpresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010DepositorInsertsAraC
alpha-HL
mScarletI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G,P-J23106-geneIII
Plasmid#80861PurposePhagemid (encodes evolved cI5G6G,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6G,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLO99
Plasmid#90219PurposeComplements mutations in Aspergillus nidulans gene lysB (AN5206) (mutants require lysine)DepositorInsertATET_03691
UseAspergillusTagsExpressionMutationPromoterpLacAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLO101
Plasmid#90221PurposeComplements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)DepositorInsertATET_07102
UseAspergillusTagsExpressionMutationPromoterpLacAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLO102
Plasmid#90222PurposeComplements mutations in Aspergillus nidulans gene choA (AN2154) (mutants require choline)DepositorInsertATET_02347
UseAspergillusTagsExpressionMutationPromoterpLacAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-scramble
Plasmid#62285Purposeexpression of scramble sgRNA from the arabinose-inducible promoterDepositorInsertscramble sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-36
Plasmid#63825PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRoDonor
Plasmid#178773PurposeExpression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalisDepositorInsertRoCAST left end, cam, RoCAST right end from Rippkaea orientalis
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII
Plasmid#80862PurposePhagemid (encodes evolved cI5G6T,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-IRES-puro-polyA
Plasmid#43967DepositorInsertpuroR
UseGateway dest vectorTagsExpressionMutationPromoterAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEc-crRNA1
Plasmid#170088Purposeencodes E. coli type I-E single spacer arrayDepositorInsertE. coli type I-E single spacer array
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterJ23119Available sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR mCherry L1-R5
Plasmid#43963DepositorInsertmCherry
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-9
Plasmid#63818PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-1
Plasmid#63816PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR eGFPCRE L1-R5
Plasmid#43960DepositorInserteGFPCre
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR IRES L5-L4
Plasmid#43964DepositorInsertIRES
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only