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Showing: 541 - 560 of 609 results

  2. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

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  3. pYSD140

    Plasmid
    #212927
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    mRuby2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212927-plasmid-map-sequence-id-421795

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  4. pSWD95D293A

    Plasmid
    #159099
    Purpose
    CckA FRET Sensor with DITE motif mutant
    Depositor
    Seth Childers
    Article
    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do
    Insert
    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CckA D293 mutated to A293 and V199I on mRuby3 (P…
    Promoter
    Xylose
    Available since
    Nov. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159099-plasmid-map-sequence-id-316589

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  5. pMM0649

    Plasmid
    #129010
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    129010-plasmid-map-sequence-id-270698

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  6. pMM0655

    Plasmid
    #129016
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129016-plasmid-map-sequence-id-261247

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  7. pMM0654

    Plasmid
    #129015
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129015-plasmid-map-sequence-id-259463

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  8. pMM0653

    Plasmid
    #129014
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pREV1-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129014-plasmid-map-sequence-id-259453

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  9. pMM0650

    Plasmid
    #129011
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pRPL18B-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129011-plasmid-map-sequence-id-259446

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  10. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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  11. pMM0651

    Plasmid
    #129012
    Purpose
    Control for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2 reporter
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pTDH3-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129012-plasmid-map-sequence-id-259438

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  12. pcDNA3.1(+)-FluoSTEP-JNKAR

    Plasmid
    #181857
    Purpose
    Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-JNKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181857-plasmid-map-sequence-id-363201

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  13. pcDNA3.1(+)-FluoSTEP-AktAR

    Plasmid
    #181856
    Purpose
    Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-AktAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181856-plasmid-map-sequence-id-362780

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  15. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

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  16. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid
    #181858
    Purpose
    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Article
    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea
    Insert
    FluoSTEP-EKAR
    Use
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181858-plasmid-map-sequence-id-363410

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  17. pVH666

    Plasmid
    #169610
    Purpose
    Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO7-PCMVmin / PPGK / PPGK
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169610-plasmid-map-sequence-id-366627

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Showing: 541 - 560 of 609 results