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Plasmid#75143PurposeExpresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cellsDepositorInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
UseTagsHAExpressionMammalianMutationLys 64 mutated to Met; kinase inactive and F236L …PromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PBK
Plasmid#23782DepositorInsertPBK (PBK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MKNK1
Plasmid#23531DepositorInsertMKNK1 (MKNK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MKNK2
Plasmid#23735DepositorInsertMKNK2 (MKNK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-LOC649288
Plasmid#23391DepositorInsertLOC649288 (AK4P6 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB
Plasmid#92234PurposeExpress wild type prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationnonePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-D661A
Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663K
Plasmid#92239PurposeExpress R663K mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663K mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to LysinePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663Q
Plasmid#92240PurposeExpress R663Q mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663Q mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to GlutaminePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698A
Plasmid#92237PurposeExpress H698A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663A
Plasmid#92238PurposeExpress R663A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAP2K2
Plasmid#23555DepositorInsertMAP2K2 (MAP2K2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK2
Plasmid#23915DepositorInsertJAK2 (JAK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-HK1
Plasmid#23730DepositorInsertHK1 (HK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK3
Plasmid#23944DepositorInsertJAK3 (JAK3 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-JAK1
Plasmid#23932DepositorInsertJAK1 (JAK1 Human)
UseGateway donor vectorTagsExpressionMutationR647H (numbering relative to NP_002218.1)PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDK9
Plasmid#23701DepositorInsertCDK9 (CDK9 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only