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Plasmid#82385PurposesgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23108Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas27
Plasmid#82384PurposesgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23117Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK2a
Plasmid#81017PurposeE.coli BW25113 + JBEI-9310 (pA5c-MevT(O)-T21-MKco, JBx_039538) + JBEI-9314(pTrc99a-PMDsc, JBx_039366)DepositorInsertsMevTo-BBa1002-pTrc-Mkco
PMD
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
UseTagsExpressionMutationPromoterLacUV5 and Trc promoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMY07AB-ORF
Plasmid#127711PurposeLevel 0 destination vector for a coding sequence with B - C scars. Clone in with BpiIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY07CD-TER
Plasmid#127712PurposeLevel 0 destination vector for a terminator with C - D scars. Clone in with BpiI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a
Plasmid#127716PurposeLevel 1 destination vector for a transcription unit, pathway position 1. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ab
Plasmid#127717PurposeLevel 1 destination vector for a transcription unit, pathway position 2. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc
Plasmid#127718PurposeLevel 1 destination vector for a transcription unit, pathway position 3. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd
Plasmid#127719PurposeLevel 1 destination vector for a transcription unit, pathway position 4. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de
Plasmid#127720PurposeLevel 1 destination vector for a transcription unit, pathway position 5. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-ef
Plasmid#127721PurposeLevel 1 destination vector for a transcription unit, pathway position 6. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-f3
Plasmid#127722PurposeLevel 1 destination vector for a transcription unit, pathway position 7. Clone a transcription unit in with BsaI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCN-mBFP
Plasmid#63847PurposeEncodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.DepositorInsertmBFP
UseTagsExpressionBacterialMutationPromoter and RBS was swapped. His-tag was removed…PromoterAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
negActinin-sstFRET-GR
Plasmid#83417PurposeFRET-based actinin tension sensor (negative control)DepositorInsertnegActinin-sstFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCKmatBC
Plasmid#138587PurposematBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, catDepositorInsertbicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
UseSynthetic BiologyTagsExpressionBacterialMutationmatB contains native GUG start codonPromoterPlac promoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK1043
Plasmid#8835DepositorInserttobacco etch virus protease, catalytic domain
UseTagsMBP and polyarginineExpressionBacterialMutationAutolysis-resistant S219V mutantPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)
Plasmid#83947PurposegRNA cassette-carrying vector with natMX markerDepositorInsertgRNA targeting ADE2 gene
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMM585-Tier1-PhCMV-Igk
Plasmid#169486PurposeTier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).DepositorInsertPCMV-driven Igk secretion signal (IGK Human)
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only