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AAV-RO1.7-RPE65

Plasmid

#206346

Purpose

AAV plasmid expressing RPE65 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Proc Natl Acad Sci U S A. 2023 Jun 6;120

Insert

Rpe65 (Rpe65 Mouse)

Use

AAV

Tags

Expression

Mutation

450-Met (C57BL6 natural variation)

Promoter

1.7kb human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT-IRES-Puro

Plasmid

#182590

Purpose

expresses TERT in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE Hygro Flag hTERT

Plasmid

#53546

Purpose

retroviral expression of human TERT

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

TERT (TERT Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCI neo-hEST2

Plasmid

#1781

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

Plasmid

#86843

Purpose

HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)

Use

Tags

FLAG-HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous TERT promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

Plasmid

#198863

Purpose

Plasmid encoding for 2 gRNAs targeting the human TERT gene

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

TERT sgRNA (TERT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

u6

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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HR-TERT-promotor

Plasmid

#71398

Purpose

Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Tags

Expression

Mutation

Promoter

Endogenous TERT promotor

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-hTERT-IRES-hygro

Plasmid

#85140

Purpose

Lentiviral expression of hTERT, used to create immortalized cell lines

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Tags

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-Hygro

Plasmid

#195512

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn-P2A-HygroR (MLH1 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Human MLH1 del754-756

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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TERT

Plasmid

#156078

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TERT (TERT Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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xlox dNGFR-TERT

Plasmid

#69805

Purpose

MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

Depositor

David Ott

Article

Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

Insert

hTERT reverse transcriptase (TERT Human)

Use

Retroviral

Tags

none

Expression

Mutation

Promoter

MLV promoter

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only
MSCVpic2neo-hTERT-FF2

Plasmid

#164910

Purpose

Expresses hTERT cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

human telomerase reverse transcriptase (TERT Human)
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Tags

HA tag

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT_-100_-79

Plasmid

#193785

Purpose

luciferase reporter for duplicated TERT promoter

Depositor

Joseph Costello

Article

Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

TERT

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TERT K78E-IRES-Puro

Plasmid

#182591

Purpose

expresses TERT K78E in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTERT (TERT Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78E

Promoter

EF-1a

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pbabe-hTERT+p53DD

Plasmid

#11128

Depositor

Christopher Counter

Article

Kendall et al Cancer Res. 2005 Nov 1. 65(21):9824-8.

Insert

hTERT, p53 dominant negative (TERT, Trp53 Mouse, Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

p53 dominant negative (aa1-14; 303-390)

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.0-TERT G228A

Plasmid

#84926

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G228A

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.0-TERT G250A

Plasmid

#84925

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G250A

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only