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HLH Clock Pas 1-401 VenN V315R

Plasmid

#47359

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, V315R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L57E/L113E/W284A

Plasmid

#47360

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Use

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L57E/L113E/W284A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pDEST-ERBB3-v1

Plasmid

#154893

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Use

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Tags

HA-GFP1-10

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v2

Plasmid

#154894

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Use

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-ERBB2-v1

Plasmid

#154901

Purpose

Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v2

Plasmid

#154895

Purpose

Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Use

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-Tau-cYFP

Plasmid

#92205

Purpose

Retroviral overexpression vector for α-Syn/Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(156-239)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only
pBABE-α-Syn-nYFP

Plasmid

#92203

Purpose

Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

SNCA (SNCA Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-Tau-nYFP

Plasmid

#92204

Purpose

Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

MAPT (MAPT Human)

Use

Retroviral

Tags

YFP(1-155)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-R3R2

Plasmid

#105109

Purpose

create your own 2in1 constructs

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-R1R4

Plasmid

#105110

Purpose

create your own 2in1 constructs

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0151

Plasmid

#172796

Purpose

Level 0 module containing CT_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0030

Plasmid

#172777

Purpose

Level 0 module containing NT2_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0031

Plasmid

#172778

Purpose

Level 0 module containing CT_nYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS N-terminal eYFP (1-174)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0040

Plasmid

#172779

Purpose

Level 0 module containing NT2_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0041

Plasmid

#172780

Purpose

Level 0 module containing CT_cYFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS C-terminal eYFP (175-238)

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0190

Plasmid

#172815

Purpose

Level 1 module containing OEP7-mTRQ_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-OEP7-mTRQ-3U+TER35S

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0100

Plasmid

#172789

Purpose

Level 0 module containing CT_Stop

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

Spacer module with stop codons.

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0150

Plasmid

#172795

Purpose

Level 0 module containing NT2_TurboID

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

TurboID with 3XHA tag

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge