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pcDNA3-PARL-D319N-FLAG C-t

Plasmid

#191957

Purpose

Expression of Presenilin Associated Rhomboid Like (PARL) D319N mutant

Depositor

Luca Scorrano

Insert

PARL D319N (PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D319N

Promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-3x-NLS-mScarlet-AMPKα2

Plasmid

#192452

Purpose

mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)

Use

Tags

3x Nuclear localization signal (NLS) and mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Use

Tags

mScarlet fused to FKBP

Expression

Yeast

Mutation

Promoter

SP6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-TsCC(B)::KANMX

Plasmid

#176397

Purpose

Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-TsCC(B)

Use

Tags

mScarlet fused to TsCC(B)

Expression

Bacterial and Yeast

Mutation

Promoter

none

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)

Plasmid

#181861

Purpose

FluoSTEP-AKAR using mScarlet-I as the FRET acceptor.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AKAR (mScarlet-I)

Use

Tags

mScarlet-I and sfGFP(1-10)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-F-gB-BlastR [M1G]

Plasmid

#171816

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-F-gB-T2A-BlastR

Use

Gene tagging

Tags

Expression

Mutation

BlastR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-F-gB-PuroR [M1G]

Plasmid

#171812

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-F-gB-T2A-PuroR

Use

Gene tagging

Tags

Expression

Mutation

PuroR: Changed Methionin 1 to Glycine.

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA844 - hrp-1p::hrp-1mScarlet

Plasmid

#139204

Purpose

Expresses mScarlet tagged hrp-1

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-C1-WPRE

Plasmid

#159955

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-C1

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Scarlet-Borcs7-Q87X

Plasmid

#118751

Purpose

Expresses scarlet tagged Borcs7-Q87X

Depositor

Beverly Koller

Insert

Borcs7-Q87X cDNA (2010012O05Rik Mouse)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Q87X

Promoter

CAG

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL1-V5-His6

Plasmid

#67416

Purpose

Expression of hArl1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL2-V5-His6

Plasmid

#67417

Purpose

Expression of hArl2 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL5A-V5-His6

Plasmid

#67420

Purpose

Expression of hArl5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4D-V5-His6

Plasmid

#67426

Purpose

Expression of hArl4D with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only