Addgene

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pAT15416-BEAR-mScarlet-target-BFP

Plasmid

#162996

Purpose

plasmid expressing an sgRNA targeting the BEAR-mScarlet plasmid along with a TagBFP marker

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

sgRNA targeting the BEAR-mScarlet plasmid

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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BFP-KASH2

Plasmid

#187019

Purpose

Inducible disruption of LINC Complex

Depositor

Jan Lammerding

Insert

KASH2

Use

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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BFP-KASH2ext

Plasmid

#187020

Purpose

Control for inducible disruption of LINC Complex

Depositor

Jan Lammerding

Insert

KASH2ext

Use

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSR04

Plasmid

#69151

Purpose

read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP
eGFP

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

eef-1A.1 (eft-3) and rps-27

Availability

Academic Institutions and Nonprofits only

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MTK3a_022

Plasmid

#123774

Purpose

Encodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

his::GAI::tagBFP::linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MTK3a_023

Plasmid

#123775

Purpose

Encodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

his::ABI::tagBFP::linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MTK4a_016

Plasmid

#123829

Purpose

Encodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

P2a-NLS::tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MTK6_004

Plasmid

#123858

Purpose

Encodes tagBFP, thosea asigna virus 2A (T2A) and Blasticidin resistance as a multicistronic cassette to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

CMV-tagBFP-T2A-BLAST-bgPA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MTK6_014

Plasmid

#123868

Purpose

Encodes WPRE expressing tagBFP T2A Blasticidin resistance cassette without a terminator to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

WPRE-tagBFP-T2A-Blast (no terminator)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MTK234_039

Plasmid

#123914

Purpose

Encodes the spCas9 tagBFP gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ARB341

Plasmid

#124024

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::tagBFP-bgPA_R1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ARB345

Plasmid

#124028

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::tagBFP-rglPA_R2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ARB349

Plasmid

#124032

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::tagBFP-SV40_RE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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JPF0455

Plasmid

#124040

Purpose

Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-NES-tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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attB53_SNCB+_attB53

Plasmid

#183614

Purpose

RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

LaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP

Use

Rmce

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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attB53_SNCB-_attB53

Plasmid

#183615

Purpose

RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

LaG17 GFP nanobody SynNotch tTA
TRE-mCherry-SV40pA
tagBFP

Use

Rmce

Tags

Myc and human CD8a signal sequence

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CROPSeq-BFP

Plasmid

#196718

Purpose

CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Use

Tags

TagBFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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pTag-BFP-C-h-Rab11a

Plasmid

#79805

Purpose

Express TagBFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab11a (RAB11A Human)

Use

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

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MTK0_015

Plasmid

#123930

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - Insulator + tagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge