Showing: 541 - 560 of 686 results
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Plasmid#211888PurposeCENP-T[1-242] transgene integration at AAVS1 safe harbor locusDepositorInsertCENP-T[1-242] (CENPT Synthetic, Human)
UseCRISPRTagsExpressionMutationT11A, T85APromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgHTT1-7sk-sgCas9
Plasmid#190900PurposeAAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAsDepositorInsertsgRNA (HTT Human, spCas9)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailabilityAcademic Institutions and Nonprofits only -
pAAVss-U6-sgHTT51-7sk-Cas9
Plasmid#190901PurposeAAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNADepositorInsertsgRNA (Htt Mouse)
UseAAV and CRISPRTagsExpressionMutationPromoterU6 and 7skAvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1
Plasmid#190902PurposeAAV vector expressing thew GFP reporter gene and human sgHTTDepositorInsertGFP and sgHTT (HTT Human)
UseAAV and CRISPRTagsExpressionMutationPromoterEFS and U6AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9
Plasmid#190903PurposeAAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9DepositorInsertGFP, sgHTT and sgCas9 (HTT Human)
UseAAV and CRISPRTagsExpressionMutationPromoterEFS and U6AvailabilityAcademic Institutions and Nonprofits only -
pCF709
Plasmid#121891PurposeEF1a-ZIKV-protease-mTagBFP2DepositorInsertsUseLentiviralTagsExpressionMutationPromoterEF1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
pCF710
Plasmid#121892PurposeEF1a-WNV-protease-mTagBFP2DepositorInsertsUseLentiviralTagsExpressionMutationPromoterEF1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
pLY232
Plasmid#184149Purposeshort tracrRNA generator with WT anti-repeat regionDepositorInserts-tracrRNA-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-sfGFP-deadTET1CD
Plasmid#184442PurposeCatalytically DEAD TET1CD with scFv against GCN4DepositorInsertCatalytically DEAD TET1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-scFvGCN4sfGFP-VP64-GB1
Plasmid#141417PurposeExpress scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activationDepositorInsertscFvGCN4sfGFP-VP64-GB1
UseTagsHA and sfGFPExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pGG369
Plasmid#165606PurposeVector for expression of ω-dCas9 and sgRNA in E. coli: lacUV2-ω-1xFLAG-dCas9(Wt PID)-1xNLS-3xHA-1xNLS and UV5-BsaI-sgRNA (BsaI sites in place of the spacer)DepositorInsertω-1xFLAG-dCas9(PAM-interacting domain and overall coding scheme from Wt SpCas9)-1xNLS-3xHA-1xNLS and BsaI-sgRNA
UseCRISPR and Synthetic BiologyTags1x FLAG, 3x HA, Omega (ω) subunit of RNA polymera…ExpressionBacterialMutationRecoding of BsaI site in the AmpR cassette, two B…PromoterlacUV2 driving ω-dCas9 and UV5 driving sgRNAAvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)AvailabilityAcademic Institutions and Nonprofits only -
pCF708
Plasmid#121685PurposeEF1a-dTEV-protease-mTagBFP2DepositorInsertTEV protease
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-sfGFP-TET1CD
Plasmid#184439PurposeTET1 catalytic domain fused with sfGFP and scFv against GCN4DepositorInsertscFv-GCN4-sfGFP-TET1CD
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA
Plasmid#168290PurposeCRISPR-activator Suntag-P65-HSF1 (SPH)DepositorInsertdCas9, Suntag-P65-HSF1
UseTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS
Plasmid#79372PurposeRecruits p65-hsf1 to a compatible Cas9 protein for transcriptional activationDepositorInsertGCN4-sfGFP-p65-hsf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 541 - 560 of 686 results