-
Plasmid#89685PurposepCAG promoter driving mtagBFPDepositorInsertmtagBFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-ACTB-C1
Plasmid#169796PurposeExpresses Cas9 and guide RNA for ACTB gene in humanDepositorInsertguide RNA for ACTB gene
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ231 - Phsp Cas9
Plasmid#191382PurposeHeat shock inducible Cas9 expression in C. elegans.DepositorInsertPhsp-16.41 Cas9 gpd-2 tagRFP-t
UseCRISPRTagsExpressionWormMutationPromoterPhsp-16.41Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hPAX7 (#348)
Plasmid#78337PurposeOverexpression of human PAX7 ORF with a N-terminal FLAG tagDepositorInsertPAX7 (PAX7 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-ACTB-C200-GFPfr1
Plasmid#169798PurposeTHe donor vector for the ACTB gene locus.DepositorInsertDonor sequence to ACTB gene for HR
UseHr donor vector for human actb gene.TagsExpressionMutationPartial sequence of GFP is inserted before the st…PromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHN922
Plasmid#178975PurposeExpression of GST-His8-GFP-nanobodyDepositorInsertGST-His8-GFP-nanobody
UseAffinity Reagent/ AntibodyTagsGST, His8ExpressionBacterialMutationPromotertacAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-TMEM106b-mApple
Plasmid#179385PurposeLentiviral vector for expression of TMEM106b fused mAppleDepositorInsertTMEM106b - mApple
UseLentiviralTagsmAppleExpressionMammalianMutationPromoterEF1Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pEGFP-Rab21-Q76L (CA)
Plasmid#83422PurposeMouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationResidue 76 from Q to L (Q76L: CAG -> CTG)PromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-ATP
Plasmid#193818PurposeCMV overexpression of ChemoG-ATP in mammalian cell linesDepositorInsertChemoG-ATP
UseTagsExpressionMammalianMutationEGFP:A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3CG
Plasmid#174189PurposeExpresses PIK3CG in mammalian cellsDepositorInsertPIK3CG (PIK3CG Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
RHCglo
Plasmid#80169PurposeMinigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicingDepositorInsertsegments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Wt1
Plasmid#41082DepositorInsertWt1 (-KTS alternative isoform) (Wt1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTetO + minimal CMVAvailable sinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pET-CjCas9
Plasmid#89754PurposeExpression of CjCas9 with His tag in E.coliDepositorInsertCjCas9
UseCRISPRTags6XHis, HA, and SV40 NLSExpressionBacterialMutationPromoterT7Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Arp3-pmCherryC1
Plasmid#27682DepositorInsertArp3 (ACTR3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Cre
Plasmid#24704DepositorInsertCre
UseTagsExpressionMammalianMutationPromoterGFAP (glial fibrillary acidic protein)Available sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSpot-Tag-GFP-GPI
Plasmid#117061PurposeFor mammalian expression of GPI-anchored GFP with N-terminal Spot-TagDepositorInsertGPI-anchored GFP
UseTagsGPI-anchor and Spot-TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag(22aa)
Plasmid#215745PurposeExpresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkersDepositorInsertdCas9-10xSunTag
UseTagsExpressionMammalianMutationPromoterchicken beta-actin promoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-mTrim21
Plasmid#104971PurposeLentiviral Transfer Plasmid with mCherry-tagged mouse Trim21DepositorInsertTripartite Motif containing 21 (Trim21 Mouse)
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-hPAC-FLAG
Plasmid#129494PurposeExpresses human TMEM206 tagged at the C-terminus with FLAGDepositorInserthuman PAC tagged with FLAG tag at the C-terminal end (PACC1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28HIS-hAPE1
Plasmid#70757Purposebacterial expression of human APE1DepositorInsertAPEX1 (APEX1 Human)
UseTags6-HISExpressionBacterialMutationPromoterT7/lac operonAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec23A
Plasmid#66609PurposeGFP fusion of Sec23ADepositorInsertSec23A (SEC23A Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis
Plasmid#156948PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNCAM1 (NCAM1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRZBA-c400
Plasmid#205109PurposeFRZB protein expressionDepositorInsertFRZB (FRZB Human)
UseTagsAviTag-6His and PTPRSspExpressionMammalianMutationWTPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(P691S)-FLAG
Plasmid#164973PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP691SPromoterEF1aAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pAM1
Plasmid#51401PurposeExpresses truncated version of NLL-MetRS from endogenous MetG promoterDepositorInsertMetRS (metG E coli)
UseTagsExpressionMutationL13N, Y260L, H301L; Please note that a truncated …PromoterMetGAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 KRAB
Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only