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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLH-nmsgRNA1.1

Plasmid

#64115

Purpose

Vector for expression of Nm sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm sgRNA1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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dCas13d-dsRBD

Plasmid

#154938

Purpose

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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HaloTag-bActinCDS-bActinUTR-MS2V5

Plasmid

#102718

Purpose

Halo-Actin reporter for detection of both mRNA and protein

Depositor

Robert Singer

Article

Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

HaloTag-bActinCDS-bActin3'UTR-MS2V5

Use

Lentiviral

Tags

HaloTag and MS2V5

Expression

Mutation

Promoter

UbC

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Use

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFCcGi

Plasmid

#59324

Purpose

Inducible expression of GFP and constitutive expression of mCherry

Depositor

David Holden, Sophie Helaine

Article

Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

Inserts

mCherry
EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pbad and rpsM

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-D1R

Plasmid

#89160

Purpose

Express vaccinia virus D1R in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D1R

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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4xMS2-hTR

Plasmid

#154940

Purpose

4xMS2 stem-loop-hTR-500 bp genomic sequence

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

4xMS2 stem-loop-hTR-500 bp genomic sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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LV-RFP

Plasmid

#26001

Depositor

Elaine Fuchs

Article

Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

Insert

H2B-RFP (H2BC21 Human, A. victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pT7-VP1SA11

Plasmid

#89162

Purpose

Express Rotavirus SA11 VP1 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-VP2SA11

Plasmid

#89163

Purpose

Express Rotavirus SA11 VP2 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-VP3SA11

Plasmid

#89164

Purpose

Express Rotavirus SA11 VP3 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP3

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7 promotor

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbOcIgG-Tn5

Plasmid

#184285

Purpose

Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

Ivan Raimondi, New York Genome Center

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbOcIgG-Tn5

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

Ivan Raimondi, New York Genome Center

Article

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_TAF15

Plasmid

#26379

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only