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Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/UBC9
Plasmid#20082DepositorInsertUBC9 (UBE2I Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-N-FLAG-HA-TBK1
Plasmid#131791PurposeLentiviral vector expressing WT TBK1DepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsN-FLAG-HAExpressionMammalianMutationWT full lengthPromoterCMVAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-FLIM-AKAR
Plasmid#60445PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKAR
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Actin-7
Plasmid#56421PurposeLocalization: Actin, Excitation: 488, Emission: 507DepositorInsertActin (ACTB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTARA
Plasmid#31491DepositorInsertRNA polymerase
UseTagsExpressionBacterialMutationN823DPromoterAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABe-HA-ER-IPpoI
Plasmid#32565DepositorInsertI-PpoI
UseRetroviralTagsER and HAExpressionMammalianMutationnonePromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CROPseq-multi-Zeo (pRW1445)
Plasmid#216218PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Zeocin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-C1
Plasmid#54653PurposeLocalization: C1 Cloning Vector, Excitation: 554, Emission: 581DepositorTypeEmpty backboneUseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa
Plasmid#127389PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alphaDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-lck-GCaMP6f
Plasmid#52924PurposeFluorescent reporter for calcium signalingDepositorHas ServiceAAV5Insertlck-GCaMP6f
UseAAVTags6xHisExpressionMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationPromoterT7Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Bxb1o_nlsFlag
Plasmid#119901PurposeExpresses Bxb1 integrase in mammalian cellsDepositorInsertMammalian codon optimized Bxb1 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tau (P301L)-VC
Plasmid#87633Purposeexpresses hTau fused to the C-terminal part of VenusDepositorInsertMAPT (MAPT Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only