-
Plasmid#123910PurposeEncodes the spCas9 sgRNA1 hCLYBL (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hCLYBL
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK7_002
Plasmid#123871PurposeEncodes the 5' 500 bp hRosa26 locus homology arm as a type 7 part to be used in the MTK systemDepositorInsertRA_hROSA26
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK7_011
Plasmid#123878PurposeEncodes the 5' 500 bp hThal4.10-2-gh18 locus homology arm as a type 7 part to be used in the MTK systemDepositorInsertRA_hTha5.10-2-hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_003
Plasmid#123857PurposeEncodes pCMV-Blasticidin resistance cassette as a type 6 part to be used in the MTK systemDepositorInsertCMV-Blast-bgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_014
Plasmid#123677PurposeEncodes ConLS' and short flippase recognition target (FRT) site as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS'-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-Venus-173-C
Plasmid#87617PurposeFKBP-tagged C-terminal Venus (YFP) fragment for BiFC.DepositorInsertFKBP-Venus173-C
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pSR28
Plasmid#69253Purposephlh-8::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterhlh-8Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
TU#1450_unc-53a_cDNA
Plasmid#82361Purposeexpress unc-53a cDNADepositorInsertunc-53 a isoform (unc-53 Nematode)
UseTagsExpressionWormMutationPromoternoneAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
3789 pmko-neo RNAi-GFP
Plasmid#8881DepositorInsertGFP
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd388-shLuc
Plasmid#83275PurposeshRNA targeting luciferaceDepositorInsertshRNA to Luciferase
UseRetroviralTagsExpressionMutationPromoterpUC oriAvailable sinceDec. 2, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
S8_attL1-N-term-EGFP-attL2
Plasmid#128472PurposeEGFP entry clonesDepositorInsertEGFP entry clones
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mesp-1916/-1>nls::Cas9::nls
Plasmid#59988PurposeMesp driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Mesp -1916/-1Available sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TOM1L2_VHS
Plasmid#109901PurposeProtein expression and purification of TOM1L2_VHSDepositorInsertTOM1L2_VHS (TOM1L2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
ARB343
Plasmid#124026PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2DepositorInsertLS-A4_EF1a-NLS::mRuby2-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB349
Plasmid#124032PurposeEncodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged tagBFPDepositorInsertL2-C3_CAG-Lyn::tagBFP-SV40_RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 DN-xIGFR
Plasmid#24699DepositorInsertDominant negative xIGFR (igf1r Frog)
UseTagsExpressionMammalianMutationC-terminal truncated version of the Xenopus IGF t…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only