Addgene

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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-SSFV-dCas9-GFP

Plasmid

#64105

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

SSFV

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRC3EGFP

Plasmid

#193790

Purpose

Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PR promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFU-no toxin-PE

Plasmid

#24149

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

PDGF-receptor-transmembrane domain (PDGFRB Aequorea victoria, Human)

Use

Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2010

Availability

Academic Institutions and Nonprofits only
pMB63

Plasmid

#47945

Purpose

Expresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and egl-13 NLS

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pICH51277

Plasmid

#50268

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAG374-H2B-greenFAST

Plasmid

#160359

Purpose

Expresses H2B-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

H2B-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-4u

Plasmid

#13553

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T163A

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T163A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL80

Plasmid

#138396

Purpose

RMCE vector for generating C-terminal T2A-GAL80 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL80

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM236 - pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Plasmid

#159797

Purpose

Histamine based negative selection marker to paralyze animals with arrays

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

Insert

pEXP[Pmlc-2 | histamine | rpl-3 UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pATD

Plasmid

#141012

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HSV-TK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dub3 mouse

Plasmid

#72682

Purpose

mammalian expression of mouse Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pME-nlsGFP-P2A

Plasmid

#80808

Purpose

nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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Dlx6

Plasmid

#15539

Depositor

John Rubenstein

Article

Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

Insert

Dlx6 (Dlx6 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pFU-MVIIA-PC

Plasmid

#24148

Depositor

Ines Ibanez-Tallon

Article

Auer et al Nat Methods. 2010 Feb 7. ():.

Insert

omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-mCherry (PDGFRB Human, Conus magus, Aequorea victoria)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2010

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPd_PepTSt

Plasmid

#58333

Purpose

Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead

Article

Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

Insert

PepTSt

Use

Tags

GFP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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