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Plasmid#89364PurposeA lenti-viral vector expressing photoswitchable Raf1 in mammalian cellsDepositorInsertpsRaf1 (RAF1 Human, Synthetic)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
human p53-(311-393)
Plasmid#24869DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains aa 311-393.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NPHS-bio-His
Plasmid#53334PurposeExpresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNPHS (NPHS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-OSH2-2xPH
Plasmid#161987PurposeMammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFPDepositorInsertOSH2 (OSH2 Budding Yeast)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-mScarlet-Frame1-Parental-Minicircle
Plasmid#216125PurposeParental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.DepositorInsertsgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only