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Plasmid#60224PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.DepositorInsertsUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS, No promoter, and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only
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lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR2
Plasmid#161753PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase A activity in living cells and in vivo.DepositorInsertExRai-AKAR2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG015 (RADAR)
Plasmid#157893PurposeExpresses the RADAR defense systemDepositorInsertRADAR subtype containing auxillary gene rdrD
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG016 (ApeA)
Plasmid#157894PurposeExpresses the ApeA defense systemDepositorInsertApeA (putative HEPN RNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG023 (dsr2)
Plasmid#157901PurposeExpresses the dsr2 defense systemDepositorInsertdsr2 (SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH94
Plasmid#91720PurposeIntegration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-GuerinDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only