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pDD379

Plasmid

#91834

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-GSK3B_IDG-K

Plasmid

#158166

Purpose

Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-GSK3B (GSK3B Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Use

Tags

mVermilion

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pC-PTP-NEO

Plasmid

#51710

Purpose

contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Article

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Expression

Mutation

Promoter

Available since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

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pCR1054

Plasmid

#71822

Purpose

Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTK_Snai2_enh_13_Citrine

Plasmid

#130574

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Snai2_enh_13

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_84_Citrine

Plasmid

#130584

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_84

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-iRFP

Plasmid

#64887

Purpose

Marking retinal neurons with infrared fluorescent proteins (iRFP) to preserve photoresponsiveness

Depositor

Tomomi Ichinose

Article

Fyk-Kolodziej et al Biotechniques. 2014 Nov 1;57(5):245-53.

Insert

iRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pVIPR2-ERT2CreERT2pA

Plasmid

#163628

Purpose

Heart-specific tamoxifen-dependent Cre expression

Depositor

Daekee Lee

Article

Chin et al Lab Anim Res. 2020 Sep 15;36:31. doi: 10

Insert

Cre recombinase

Use

Cre/Lox

Tags

ERT2 on both N-terminal and C-terminal of Cre

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-1st WW mutant

Plasmid

#19046

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein isoform 3 (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
Myc-PSFΔRRM2

Plasmid

#35377

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 375-429

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK20

Plasmid

#59120

Purpose

FAK fret sensor with 20 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo-HA-hUVRAG

Plasmid

#24290

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV irradiation resistance-associated gene (UVRAG Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
pYpet-FsaA

Plasmid

#98279

Purpose

mammalian expression of Ypet-FsaA

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FsaA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Use

Tags

MBP

Expression

Bacterial

Mutation

H759A

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1

Plasmid

#35376

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-365

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
TALED_Left-stuffer-8e-E1347A

Plasmid

#187163

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Left-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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