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pEGFP-N1-mSNX27-ΔF3

Plasmid

#163618

Purpose

mouse SNX27 without the F3 domain cDNA C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-ΔF3 (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

deletion F3 domain

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Daniel Hammer, Matthew Good, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Use

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV.5’-LMNA-eGFP-KI

Plasmid

#170545

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for LMNA knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’VIM-eGFP-KI

Plasmid

#170547

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Inserts

Left Homology arm for VIM knockin
Right Homology arm for VIM knockin

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Use

Tags

mGFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
14UAS zfPSD95:GFP

Plasmid

#74314

Purpose

A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

Depositor

Stephen Smith, Martin Meyer

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Insert

zebrafish PSD95

Use

Tags

EGFP

Expression

Mutation

Y599H and I793S (please see depositor comment bel…

Promoter

UAS

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pLenti HsATP10B - EmGFP

Plasmid

#204474

Purpose

transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

Depositor

Veerle Baekelandt

Article

Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

Inserts

ATP10B (ATP10B Human)
EmGFP

Use

Lentiviral

Tags

EmGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Use

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsY14_I

Plasmid

#146583

Purpose

Mammalian Expression of HsY14

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsY14 (RBM8A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-EGFP-ccdB

Plasmid

#14316

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-fCR-GFP

Plasmid

#22911

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu calretinin promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only
PdnaK GFP pZa

Plasmid

#170073

Purpose

GFP expression under the control of E. coli dnaK promoter

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PdnaK

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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paGFP-HRas G12V

Plasmid

#18694

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

H-Ras G12V (HRAS Human)

Use

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP G12V mutation in HRas

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pWL078 MCP-2xGFP

Plasmid

#197047

Purpose

pCYC-NLS-MCP-2xGFP on pRS305

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

MCP-2xGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CYC1

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-2A-TAL6-MSTN

Plasmid

#72597

Purpose

MSTN TALEN with 2A-GFP tagged

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

TALEN for MSTN

Use

TALEN

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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