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Showing: 5521 - 5540 of 54106 results

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pGL3-U6-pegRNA_HOXD13_CtoT-EGFP

Plasmid

#159792

Purpose

S. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_HOXD13_CtoG-EGFP

Plasmid

#159791

Purpose

S. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
TadA-NG-CBE

Plasmid

#227420

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

TadA-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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YEE-NG-CBE

Plasmid

#227418

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

rA1-YEE-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDON223 Spike D138Y

Plasmid

#180056

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D138Y (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D138Y

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A570D

Plasmid

#180064

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A570D (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A570D

Promoter

Available since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike H655Y

Plasmid

#180059

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike H655Y (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike H655Y

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike S982A

Plasmid

#180067

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike S982A (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike S982A

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike T1027I

Plasmid

#180058

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike T1027I (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike T1027I

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike D1118H

Plasmid

#180068

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D1118H (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D1118H

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike K417N

Plasmid

#180060

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike K417N (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike K417N

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike A701V

Plasmid

#180062

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A701V (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A701V

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Use

Tags

NLS

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Rac1

Plasmid

#180575

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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