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Plasmid#22263DepositorInsertcyclin-dependent kinase inhibitor 2A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC57-rm_gCarvi-T2A
Plasmid#190334PurposeRatiometric reporter for cAMPDepositorInsertratiometric gCarvi
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311
Plasmid#113668PurposeGateway destination vector for lentiviral expressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M1
Plasmid#23007DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 R62D actin-3XNLS P2A mCherry
Plasmid#58477PurposeExpresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsUseTagsmCherryExpressionMammalianMutationChanged Arginine 62 to Aspartic AcidPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-(mNeonGreen)4-tDeg
Plasmid#129402Purpose(mNeonGreen)4-tDeg fluorogenic proteinDepositorInsert(mNeonGreen)-tDeg
UseTagsExpressionMammalianMutationPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCherry LIC cloning vector (6B)
Plasmid#30125PurposeEmpty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
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PCDNA3 Flag p38 alpha (agf)
Plasmid#20352DepositorInsertpDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorInsertZdk2
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Factor IX donor
Plasmid#182141PurposeFactor IX donor for twinPE and Bxb1 mediated insertion at ALBDepositorInsertattP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-G_oT7pol-IRES-BSD
Plasmid#172369PurposeGeneration of oT7pol-expressing stable cell lines, resistant to the antibiotic BlasticidinDepositorInsertoT7pol + BSD
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-mRuby3
Plasmid#74252PurposeMammalian expression of tandem fusion of mClover3-mRuby3DepositorInsertmClover3-mRuby3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NDP52
Plasmid#135296Purposestably express GFP-tagged NDP52 for CID induced mitophagyDepositorInsertNDP52 (CALCOCO2 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-C CRISPR
Plasmid#140647PurposeCTCF tagging CRISPRDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EB3-tdTomato
Plasmid#50708Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTsDepositorInsertEB3 (MAPRE3 Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACBSR-hyg
Plasmid#87830PurposeExpresses lambda-red system with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterblaAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
MMLV-CAG-SADB19_oG-IRES-Puro
Plasmid#172367PurposeGeneration of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic PuromycinDepositorInsertoG + Puro
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
UseTagsAID(71-114)ExpressionYeastMutationPromoterADH1 and TEF1Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 D18N BLAST
Plasmid#164225Purposelentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationD18NPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet3_N1
Plasmid#189778PurposeConstruct for testing oligimerization of mScarlet3 in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF_PE2
Plasmid#199267PurposeMammalian expression of SpCas9 prime editor 2 under EF1α promoterDepositorInsertPE2-P2A-mp53DD
UseTagsExpressionMammalianMutationΔ40-903PromoterAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3347
Plasmid#144823PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPITX1 (PITX1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DHOV_GP
Plasmid#207267PurposeMammalian expression of Dhori virus GP proteinDepositorInsertDHOV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only