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Plasmid#190350PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-min35S::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSA
Plasmid#195207PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphorylation-deficient mutant (S162/339/340A)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340APromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 3xSD
Plasmid#195208PurposeMammalian expression vector for GFP tagged NHERF1. Triple PKC site phosphomimetic mutant (S162/339/340D)DepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationS162/339/340DPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 PDZ1*
Plasmid#195210PurposeMammalian expression vector for GFP tagged NHERF1. PDZ1 GYGF>GYAA motif mutantDepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationPDZ1 GYGF>GYAAPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-NHERF1 PDZ2*
Plasmid#195212PurposeMammalian expression vector for GFP tagged NHERF1. PDZ2 GYGF>GYAA motif mutantDepositorInsertNHERF1 (NHERF1 Human)
UseTagsExpressionMammalianMutationPDZ2 GYGF>GYAAPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPCNA-S228I
Plasmid#190938Purposeuntagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsExpressionBacterialMutationchanged serine 228 to isoleucinePromoterT7Available sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL65
Plasmid#175891PurposepENTRY for Gateway LR reactionDepositorInsertID#11
UsePentry cloneTagsExpressionMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c011
Plasmid#175454PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649. Can be biotinylated.DepositorInsertCD44 (CD44 Human)
UseTagsAviTag-His6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD44U-c010
Plasmid#175453PurposeProtein expression/secretion in mammalian cells. CD44 splice variant 10 (exons 2-5, 11, 15-16), A20-E649.DepositorInsertCD44 (CD44 Human)
UseTagsHis6ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A149)
Plasmid#165998PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A149)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(E167)
Plasmid#166004PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(E167)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(D165)
Plasmid#166003PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(D165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(C164)
Plasmid#166002PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(C164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(N180)
Plasmid#166000PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(N180)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A165)
Plasmid#165999PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A165)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-HisDAD2 cPGFP(G164)
Plasmid#165997PurposeExpression of coding sequence in Escherichia coliDepositorInsertHisDAD2 cPGFP(G164)
UseTags6HisExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rShHTL7 cPGFP(L166)
Plasmid#165995PurposeExpression of coding sequence in Escherichia coliDepositorInsertrShHTL7 cPGFP(L166)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(G164)
Plasmid#165993PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(G164)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
Plasmid#162811PurposeControl STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.DepositorInsertBsmBI_(C1A)-Intein_DD
UsePiggybacTagsExpressionMammalianMutationIntein(Cys1,Ala)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
Plasmid#162814PurposeSTELLA plasmid for amber suppression of C-terminally labelled SMIM38DepositorInsertSMIM38(C-TAG)-Intein-DD
UsePiggybacTagsExpressionMammalianMutationSMIM38(Ochre52,Amber)PromoterEF1alphaAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only