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Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterU6 (for expressing sgRNA)Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only
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p2R3z-BASI-ccdB-BSAI
Plasmid#118389PurposeEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-bJunVN155(I152L)
Plasmid#27098DepositorInsertbJunVN155(I152L) (Jun and Aequorea Victoria, Rat)
UseTagsMycExpressionMammalianMutationI152L in Venus(1-154)PromoterAvailable sinceJan. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
Plasmid#87693PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Gal4VP16-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE-TO-Sp dCas9-mVenusN
Plasmid#191396PurposeSpdCas9 fused to N-terminal part of mVenusDepositorInsertN-terminal part of split mVenus
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C3-HA-Ubiquitin
Plasmid#28236DepositorInsertUbiquitin (UBB Human)
UseTagsEYFP and HAExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1-14-mVenusC
Plasmid#191393PurposeMBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mem
Plasmid#55779PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.DepositorInsertmCherry-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
neurod1:CaMPARI
Plasmid#173479PurposeCaMPARI is driven by -5kb neurod1 promoter.DepositorInsertCaMPARI
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
3120 pSFFV-neo Bcl-XL
Plasmid#8749DepositorInsertBcl-xl (BCL2L1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK7-FLAG-TSC2
Plasmid#8996DepositorInsertTSC2 (TSC2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAW-AlphaLobe
Plasmid#113255PurposeE. coli expression vector for His-MBP-Cas9 Alpha-Helical LobeDepositorInsertS. pyogenes Cas9 alpha-helical lobe
UseTags10xHis-MBPExpressionBacterialMutationresidues 56–714; See paper for descriptionPromoterT7Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ran-T24N-mRFP1-polyA
Plasmid#104561PurposeExpress Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorInsertRan (Ran Mouse)
UseTagsmRFP1ExpressionMammalianMutationT24NPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha WT
Plasmid#16390DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-Hs beta1
Plasmid#45927DepositorInsertHs Beta1 adaptin (AP1B1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(-30)GFP
Plasmid#62936PurposeExpression of 6xHis-(-30)GFP in bacterial cellsDepositorInsert(-30)GFP
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL2620 (pTniQ_Vch)
Plasmid#200892PurposeExpresses human codon-optimized V. cholerae TniQ from a CMV promoter.DepositorInsertVchTniQ
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya
Plasmid#186980Purposemammalian expression of Cluc-Gluc reporterDepositorInsertCluc, Gluc
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only