-
Plasmid#214657Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0527
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 specR cassette
Plasmid#117120PurposeSpectinomycin resistance cassetteDepositorInsertSpectinomycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-HA-GFP11-N1
Plasmid#91953PurposeExpresses GST-HA-GFP11 in mammalian cellsDepositorInsertGST-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
yWL268 MCP-GFP
Plasmid#197052PurposepUBC-NLS-HA-2xMCP-2xGFPDepositorInsertpubc-nls-ha-MCP-2xGFP
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct-GW-HygroR
Plasmid#149610PurposeGateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.DepositorInsertGateway cassette (ccdb + Chlor.R.)
UseTagsExpressionInsectMutationPromoterAct5cAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
UseTags3xFLAG, 6xHis, and pelB leaderExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRP2365
Plasmid#127575PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPID2
Plasmid#169898PurposeMinimal empty vector with piggyBac, insulators, and a DTSDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP2370
Plasmid#127576PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV h c-MYC IRES GFP
Plasmid#18119DepositorInsertc-MYC (MYC Human)
UseRetroviralTagsGFPExpressionMutationThe CUG which is the start site for Myc I is pres…PromoterAvailable sinceJune 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-RagC
Plasmid#99718PurposeMammalian expression of HA-RagCDepositorInsertRagC (RRAGC )
UseTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
RP4-7
Plasmid#79811PurposeModified broad host range plasmid RP4. Chloramphenicol resistant. Helper plasmidDepositorInsertChloramphenicol acetyltransferase
UseHelper plasmid for mobilizationTagsExpressionMutationPromoterOwn promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_TIR1
Plasmid#119766Purposein vitro transcription for mRNADepositorInsertTIR1
UseTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
His_CL7_2NLS_iCas12a-4NLS
Plasmid#222971PurposeBacterial expression of NLS-rich iCas12a constructDepositorInsertHis_CL7_2NLS_iCas12a-4NLS
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAX-SCLM
Plasmid#216757PurposeFor strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoterDepositorInsertSuperClomeleon
UseTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCAG (cytomegalovirus-chicken beta-actin-rabbit be…Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
APEX2-ChBP
Plasmid#108878PurposeExpression of APEX2 fused to a nanobody against mCherryDepositorInsertAPEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a mScarlet
Plasmid#178543PurposeThe mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmScarlet
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAH123
Plasmid#66077PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionDepositorInsertphi80 integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TadCBE-Cas9NG
Plasmid#225093PurposePlasmid for bacterial purification of codon-optimized TadCBE-Cas9NGDepositorInsertTadCBE-Cas9NG
UseCRISPRTags8xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only