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Showing: 5461 - 5480 of 16015 results

  1. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    127177-plasmid-map-sequence-id-246566

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  2. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

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  4. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  5. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

    Enlarge

  6. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al bioRxiv 2022.12.10.519870
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  7. pMLLCK-J72030

    Plasmid
    #26172
    Depositor
    Christopher Anderson
    Insert
    C terminal E tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26172-plasmid-map-sequence-id-12293

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  8. pMLLCK-K112507

    Plasmid
    #26174
    Depositor
    Christopher Anderson
    Insert
    C terminal HA tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26174-plasmid-map-sequence-id-12295

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  9. pMLLCK-J72032

    Plasmid
    #26177
    Depositor
    Christopher Anderson
    Insert
    C terminal S tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26177-plasmid-map-sequence-id-12298

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  10. pMLLCK-J72034

    Plasmid
    #26179
    Depositor
    Christopher Anderson
    Insert
    C terminal T7 tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26179-plasmid-map-sequence-id-12300

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  11. pMLLCK-J72035

    Plasmid
    #26180
    Depositor
    Christopher Anderson
    Insert
    C terminal V5 tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26180-plasmid-map-sequence-id-12301

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  12. PsuDJ-1.1

    Plasmid
    #52080
    Purpose
    Encodes Panagrolaimus superbus DJ-1. Potent antioxidant and molecular chaperone
    Depositor
    Ann Burnell
    Insert
    PsuDJ-1.1
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  13. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  14. pLEX_306

    Plasmid
    #41391
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Availability
    Academic Institutions and Nonprofits only
    41391-plasmid-map-sequence-id-229869

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  15. pMLLKA-J72017

    Plasmid
    #26008
    Depositor
    Christopher Anderson
    Insert
    N terminal avi tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26008-plasmid-map-sequence-id-12196

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  16. pMLLKA-J72018

    Plasmid
    #26009
    Depositor
    Christopher Anderson
    Insert
    N terminal E tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26009-plasmid-map-sequence-id-12197

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  17. pMLLKA-J72019

    Plasmid
    #26010
    Depositor
    Christopher Anderson
    Insert
    N terminal Flag tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26010-plasmid-map-sequence-id-12198

    Enlarge

  18. pMLLKA-J72020

    Plasmid
    #26011
    Depositor
    Christopher Anderson
    Insert
    N terminal HA tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26011-plasmid-map-sequence-id-12199

    Enlarge

  19. pMLLKA-J72021

    Plasmid
    #26012
    Depositor
    Christopher Anderson
    Insert
    N terminal HSV tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26012-plasmid-map-sequence-id-12200

    Enlarge

  20. pMLLKA-J72023

    Plasmid
    #26014
    Depositor
    Christopher Anderson
    Insert
    N terminal S tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26014-plasmid-map-sequence-id-12202

    Enlarge

Showing: 5461 - 5480 of 16015 results