-
-
pLV/Guide-mCherry
Plasmid#170543PurposeExpresses mCherry and contains a cassette for U6 driven sgRNA transcriptionDepositorInsertmCherry
UseCRISPR and LentiviralTagsExpressionMutationPromoterEf1-alphaAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xHA-miniTurbo_pAS32
Plasmid#118222Purposeexpresses 3xHA-tagged miniTurbo in C. elegans intestineDepositorInsertminiTurbo (BirA mutant)
UseTags3x HA tagExpressionWormMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterges-1pAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His6-ODC-Ctag
Plasmid#163614PurposeExpresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.DepositorInsertODC (ODC1 Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-V5_pRS415
Plasmid#199667Purposeexpresses LOV-Turbo in the yeast cytosolDepositorInsertLOV-Turbo
UseTagsV5ExpressionYeastMutationPromoterGal1,10Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT007
Plasmid#171634PurposePlasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2DepositorInsertsspyCas9 sgRNA-TRAC
mTagBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mSin1 shRNA #1
Plasmid#13483DepositorInsertmSin1 (MAPKAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-TRPV1
Plasmid#140945PurposeQUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2ADepositorInsert15x-QUAS-dTomato-T2A-TRPV1
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-ERM
Plasmid#199664Purposeexpresses LOV-Turbo on the mammalian ER membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsSEC61B and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag WDR24 pLJM1
Plasmid#46337DepositorInsertWDR24 (WDR24 Human)
UseLentiviralTagsFLagExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO mutant ITR
Plasmid#67905PurposePiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutationDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMN-AS-HNF4α
Plasmid#139305PurposeKnocking down of human HNF4α through shRNA and amiRNA targeting HNF4αDepositorInsertshRNA and amiRNA against HNF4α
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059APromoterAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
TPI in pCW57_tTA_Blast
Plasmid#154209PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPIDepositorInsertTPI1 (TPI1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB
Plasmid#162699PurposeExpresses pHnCB10 derived carboxysome operon and prkDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW1-Myc-DJ1-WT
Plasmid#29347DepositorInsertDJ1 (PARK7 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only
