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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufCDSUB

Plasmid

#195055

Purpose

Expresses SUF operon from B. subtilis from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufCDSUB

Expression

Bacterial

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMM643

Plasmid

#112533

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Mutation

A17P

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-hSERT

Plasmid

#70103

Purpose

mammalian expression of hSERT with N-terminal YFP fusion

Depositor

Harald Sitte

Article

Sucic et al J Biol Chem. 2010 Apr 2;285(14):10924-38

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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plRL19

Plasmid

#163634

Purpose

L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Insert

L5 Integrase

Expression

Bacterial

Promoter

Mycobacterial Optimized Promoter (MOP) and Tet-On

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700

Plasmid

#64046

Purpose

Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

U6, CAGGS

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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SFRS2IP

Plasmid

#156012

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SFRS2IP (SCAF11 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufABCDSE

Plasmid

#195056

Purpose

Expresses SUF operon from E. coli from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufABCDSE

Expression

Bacterial

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-mito-mRaspberry

Plasmid

#219568

Purpose

Lentiviral construct expressing a mitochondria-targeted mRaspberry

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito (COX8A Human)

Use

Lentiviral

Tags

mRaspberry

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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CPSF5

Plasmid

#155480

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF5 (NUDT21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-3xFlag

Plasmid

#245989

Purpose

Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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HR180-LGR5-iCT

Plasmid

#129094

Purpose

KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

Depositor

Toshiro Sato

Article

Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

Insert

iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)

Use

Homologous recombination donor plasmid

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Runx2

Plasmid

#207225

Purpose

doxycycline-inducible expression of Mouse Runx2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-RUNX2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-SpCas9

Plasmid

#117051

Purpose

In vitro expression of S. pyogenes Cas9 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

S. pyogenes Cas9

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-EXO1a

Plasmid

#111629

Purpose

Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

Insert

Exonuclease 1a (EXO1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBOB-HA-Gna14

Plasmid

#246002

Purpose

Express HA N-terminal tagged mouse Galpha14 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Gna14 (Gna14 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PRPF19

Plasmid

#155847

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF19 (PRPF19 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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