Addgene

Showing: 5441 - 5460 of 14406 results

Results per page:

Sort results by:

pACYCDuet_F31YG121VDHFR_R166QTS

Plasmid

#91228

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121 and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YL54IDHFR_R166QTS

Plasmid

#91230

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_F31YL54IDHFR_WTTS

Plasmid

#91231

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

F31YL54I

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_G121VDHFR_R166QTS

Plasmid

#91232

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

G121V and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_L54IDHFR_R166QTS

Plasmid

#91234

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

L54I and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_L54IDHFR_WTTS

Plasmid

#91235

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

L54I

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYCDuet_M42FDHFR_R166QTS

Plasmid

#91236

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Use

Tags

6xHis

Expression

Bacterial

Mutation

M42F and R166Q

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_Trf2

Plasmid

#125159

Purpose

Gateway entry clone

Depositor

Alexander Stark, Julius Brennecke

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

Trf2 (Trf2 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z19

Plasmid

#124791

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z32

Plasmid

#120075

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

S-Tag

Use

Pcr template

Tags

S-Tag

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z30

Plasmid

#120073

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_Theo-LUCID

Plasmid

#119048

Purpose

Plasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Theo-LUCID_part1
Fab_Theo-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_Quin-LUCID

Plasmid

#119049

Purpose

Plasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_Quin-LUCID_part1
Fab_Quin-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO-Fab_MTX-LUCID

Plasmid

#119047

Purpose

Plasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al Angew Chem Int Ed Engl. 2017 Jun 12;56(2

Inserts

Fab_MTX-LUCID_part1
Fab_MTX-LUCID_part2

Use

Tags

FLAG-tag, His-tag, and Strep-tag

Expression

Mammalian

Mutation

Promoter

EF-1α + CMV/SV40 enhancers and IRES

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

Plasmid

#114730

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M7

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
T7T3-PacI_MafB_ISH

Plasmid

#87670

Purpose

In situ probe for mouse Mafb

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mafb (Mafb Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/T3

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPLtetO-PdhR

Plasmid

#89655

Purpose

Plasmid for titrating synthesis of PdhR with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

Pyruvate dehydrogenase complex repressor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPLtetO-FtsB

Plasmid

#107559

Purpose

Plasmid for titrating synthesis of FtsB with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pLtetO

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD-Tam1

Plasmid

#101639

Purpose

Encodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Tam1

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pThom77.6

Plasmid

#101637

Purpose

Encodes the fluorescent protein amFP486/K68M under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Use

Tags

Expression

Mammalian

Mutation

K68M

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge