Addgene

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pAG416GAL-ccdB-EGFP

Plasmid

#14195

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 EGFP-Sumo2

Plasmid

#13385

Depositor

Mary Dasso

Article

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 (SUMO2 Human)

Use

Tags

EGFP and GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(T217A)

Plasmid

#187025

Purpose

Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Alanine

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-S63C-GFP

Plasmid

#78911

Purpose

Mammalian expression of BAP1 with S63E and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

S63C

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-3C-FIP200-GFP

Plasmid

#187832

Purpose

Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and mGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 . 8():49.

Insert

SYN-EGFP-WPRE (SYN1 Human, jellyfish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-p50

Plasmid

#107977

Purpose

Expresssion of NF-kB p50

Depositor

Georg Weber

Insert

NF-kB p50 (Nfkb1 Mouse)

Use

Tags

N/A

Expression

Mammalian

Mutation

Amino acids VQACI added to C-terminus

Promoter

CMV

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rim2

Plasmid

#170869

Purpose

Mammalian expression of His-GFP-Rim2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-rtTA

Plasmid

#32648

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
rtTA

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-HLA-A2_GFP

Plasmid

#85161

Purpose

HLA.A2_GFP fusion with 2 glycines in between

Depositor

Tone Fredsvik Gregers, Sébastien Walchli

Article

Walchli et al Eur J Immunol. 2014 Mar;44(3):774-84. do

Insert

HLA.A2 (HLA-A Human)

Use

Gateway entry vector

Tags

EGFP

Expression

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-RIAM(1-666)

Plasmid

#80028

Purpose

Mammalian expression of GFP-RIAM(1-666)

Depositor

Chinten James Lim

Article

Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

Insert

RIAM (APBB1IP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-666

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pOPTN1-424-EGFP

Plasmid

#68840

Purpose

deleted OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-424 only

Promoter

CMV

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
NET33 pEGFP-N2 (597)

Plasmid

#61989

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET33 (SCARA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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FCK-Chronos-GFP

Plasmid

#80902

Purpose

Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Tubulin-6

Plasmid

#57159

Purpose

Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
BiDi-[mCherry2]mEGFP

Plasmid

#182874

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mEGFP, mCherry2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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RGD-sfGFP-I27-CC

Plasmid

#85392

Purpose

MTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labeling

Depositor

Khalid Salaita

Article

Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

Insert

RGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV sMTase (eGFP)

Plasmid

#89930

Purpose

Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

dCas9-MC-IRES-MN

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

deactivated Cas9

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPAN3

Plasmid

#37374

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsPAN3 (PAN3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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