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Plasmid#49347PurposeThis is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.DepositorInsertBmp2 promoter (shorter sequence) (BMP2 Human)
UseLuciferaseTagsluciferaseExpressionMutationPromoterBmp2 promoterAvailable sinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLIB_GST-nsp13 (SARS-CoV-2)
Plasmid#169190PurposeBaculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cellsDepositorInsertGST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
UseTagsGSTExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQhsCDtRNA-
Plasmid#86015PurposeMulti-gene expression system with human box C/D proteins with U14DepositorInsertsUseCo-expression; purificationTags6x-HisExpressionBacterialMutationamino acids 1-401, amino acids 1-411, and amino a…PromoterAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
Plasmid#72608PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine37 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDual
Plasmid#207883PurposeExpresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.DepositorInsertsThermosensitive fusion of I-OnuI and the L212P VMA1 intein.
Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
UseSynthetic BiologyTagsExpressionBacterialMutationLeucine 212 changed to proline, leucine 434 in th…PromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR
Plasmid#224435PurposeAAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.DepositorInsertsmScarlet-KASH
U6-gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES
Plasmid#182433PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertsVP2C-FPPS
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (M)
Plasmid#182435PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed VLP NES (G)
Plasmid#182477PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)
deltaVP1
VP2C-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PMP34-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170997PurposeHiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)DepositorInsertPMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianMutationPromoterEf1-alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C1ORF43(1-41)-3xFLAG
Plasmid#130881PurposeExpresses first 41 amino acids of human C1ORF43 with C-terminal 3xFLAG tagDepositorInsertC1ORF43 (C1orf43 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationDeleted amino acids 44-253PromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA4
Plasmid#89722PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA4 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335A_hCas9(D10A)_IRF8gRNA3
Plasmid#89721PurposeKnockout IRF8 in human cellsDepositorInsertIRF8 gRNA3 (IRF8 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
Plasmid#72609PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged threonine34 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S33A-GATA6-3XAU1 puro
Plasmid#72610PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro
Plasmid#72611PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37DepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33, threonine34 and serine37 to ala…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits