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Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorInsertXRCC4 (XRCC4 Human)
UseTagsGFP-2xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pCDNA3
Plasmid#107173Purposeexpresses V5-tagged TurboID on the mammalian ER membraneDepositorInsertTurboID (BirA mutant)
UseTagsExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP-5UAS DsRedExpress
Plasmid#74317PurposeA fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafishDepositorInsertssynaptophysin
DsRedExpress
UseTagsEGFPExpressionMutationPromoter5UASAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralTagsExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
ML1Nwt_in_pEGFP-C1
Plasmid#67797PurposeMammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFPDepositorInsertML1N*2-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-Ku70
Plasmid#46957PurposePlasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.DepositorInsertKu70 (XRCC6 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pCDNA3
Plasmid#107174Purposeexpresses V5-tagged miniTurbo on the mammalian ER membraneDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-miniTurbo-V5_pLX304
Plasmid#107176Purposeexpresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vectorDepositorInsertminiTurbo (BirA mutant)
UseLentiviralTagsExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEVExpressionBacterialMutation∆165NPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
NET47 pEGFP-N2 (593)
Plasmid#61986Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET47 (TM7SF2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV SOCS-3
Plasmid#11486DepositorInsertSOCS-3 (Socs3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTarget
Plasmid#186452Purposeused as source for acquisition of new spacersDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Puro-iDEST
Plasmid#75336PurposeGATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expressionDepositorInsertTRE-DEST
UseTagsExpressionMammalianMutationPromoterTRE-tightAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nHA Spinophilin
Plasmid#87122Purposemammalian expression of SpinophilinDepositorInsertSpinophilin (PPP1R9B Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only