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Showing: 10801 - 10840 of 39886 results

  1. Anti-GluA2/GluR2 glutamate receptor [L21/32R-rat IgG2a]

    Plasmid
    #222179
    Purpose
    Mammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody. (Gria2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222179-plasmid-map-sequence-id-437535

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  2. Anti-GluN2B/NR2B glutamate receptor [N59/20R-rat IgG2a]

    Plasmid
    #222186
    Purpose
    Mammalian Expression Plasmid of anti-GluN2B/NR2B glutamate receptor (Rat). Derived from hybridoma N59/20.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody. (Grin2b Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222186-plasmid-map-sequence-id-437507

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  4. Anti-GluN3A/NR3A glutamate receptor [N416/40R]

    Plasmid
    #211587
    Purpose
    Mammalian Expression Plasmid of anti-GluN3A/NR3A glutamate receptor (Rat) IgG2a R-mAb. Derived from hybridoma N416/40.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-GluN3A/NR3A glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody. (Grin3a Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211587-plasmid-map-sequence-id-418252

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  5. Anti-GluA2/GluR2 glutamate receptor [L21/32R-1]

    Plasmid
    #206693
    Purpose
    Mammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32-1.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant Mouse monoclonal antibody (Gria2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206693-plasmid-map-sequence-id-414106

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  7. Anti-Slo1/BKAlpha maxi-K+ channel [L6/48R]

    Plasmid
    #206523
    Purpose
    Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/48.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant Mouse monoclonal antibody (Kcnma1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206523-plasmid-map-sequence-id-405719

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  8. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b]

    Plasmid
    #199406
    Purpose
    Mammalian expression plasmid of Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b] (Rat). Derived from hybridoma L6/60.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Slo1/BKAlpha maxi-K+ channel (Rat) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    199406-plasmid-map-sequence-id-395978

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  9. Anti-LRRK2/Dardarin, N3 [N231B/34R]

    Plasmid
    #206543
    Purpose
    Mammalian Expression Plasmid of anti-LRRK2/Dardarin, N3 (non-mouse-reactive) (Human). Derived from hybridoma N231B/34.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-LRRK2/Dardarin, N3 (non-mouse-reactive) (Homo sapiens) recombinant Mouse monoclonal antibody (LRRK2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206543-plasmid-map-sequence-id-429353

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  10. Anti-Cav1.2 Ca2+ channel phosphorylation site pS1928 [L132/18R]

    Plasmid
    #188193
    Purpose
    Mammalian Expression Plasmid of anti-Cav1.2 Ca2+ channel phosphorylation site pS1928 (Rat). Derived from hybridoma L132/18
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Cav1.2 Ca2+ channel phosphorylation site pS1928 (Rat) recombinant mouse monoclonal antibody (Cacna1c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    188193-plasmid-map-sequence-id-377192

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  11. Anti-Thyroid hormone receptor beta1 [N402/13]

    Plasmid
    #190322
    Purpose
    Mammalian Expression Plasmid of anti-Thyroid hormone receptor beta1 (Human). Derived from hybridoma N402/13.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Thyroid hormone receptor beta1 (Homo sapiens) recombinant Mouse monoclonal antibody (THRB Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190322-plasmid-map-sequence-id-376329

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  12. Anti-Thyroid hormone receptor beta1 [N402/46]

    Plasmid
    #190323
    Purpose
    Mammalian Expression Plasmid of anti-Thyroid hormone receptor beta1 (Human). Derived from hybridoma N402/46.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Thyroid hormone receptor beta1 (Homo sapiens) recombinant Mouse monoclonal antibody (THRB Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190323-plasmid-map-sequence-id-376312

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  13. Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R]

    Plasmid
    #177539
    Purpose
    Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human). Derived from hybridoma N37A/10.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177539-plasmid-map-sequence-id-348553

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  14. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Expression
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131005-plasmid-map-sequence-id-305005

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  16. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  18. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid
    #183080
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -124C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183080-plasmid-map-sequence-id-363419

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  19. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

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  20. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  23. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131461-plasmid-map-sequence-id-305184

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  25. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  26. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131463-plasmid-map-sequence-id-305001

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  27. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183079-plasmid-map-sequence-id-363414

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  28. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid
    #129420
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    TagBFP2-V5 tag
    Expression
    Mutation
    G12V
    Promoter
    none (3x polyA to mitigate episomal expression)
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129420-plasmid-map-sequence-id-257068

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  29. Anti-Cav1.2/1.3 Ca2+ channel [L57/46R]

    Plasmid
    #177485
    Purpose
    Mammalian Expression Plasmid of anti-Cav1.2/1.3 Ca2+ channel (Rabbit). Derived from hybridoma L57/46.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Cav1.2/1.3 Ca2+ channel (Oryctolagus cuniculus) recombinant mouse monoclonal antibody (CACNA1C, CACNA1D Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    177485-plasmid-map-sequence-id-347921

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  30. Anti-GAD65/GAD67 [L127/12-rat IgG2a] - Chimeric

    Plasmid
    #227046
    Purpose
    Mammalian expression plasmid of anti-GAD65/GAD67 (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L127/12.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-GAD65/GAD67 (Homo sapiens) recombinant mouse monoclonal antibody (GAD1, GAD2 Chimera: M.musculus (mouse)/R.norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    227046-plasmid-map-sequence-id-459983

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  31. Anti-FGF14/FHF4 [N56/21R-rat IgG2a] - Chimeric

    Plasmid
    #231830
    Purpose
    Mammalian expression plasmid of anti-FGF14/FHF4 (Mouse) rat IgG2a R-mab. Derived from hybridoma N56/21.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-FGF14/FHF4 (Mus musculus) recombinant mouse monoclonal antibody (Fgf14 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231830-plasmid-map-sequence-id-459977

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  32. Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-rat IgG2a] - Chimeric

    Plasmid
    #231840
    Purpose
    Mammalian expression plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) rat IgG2a R-mab. Derived from hybridoma N37A/10.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody (KCNQ1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    231840-plasmid-map-sequence-id-459052

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  33. Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-rat IgG2a]

    Plasmid
    #222180
    Purpose
    Mammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody. (Kcnma1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222180-plasmid-map-sequence-id-437503

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  34. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid
    #183078
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    V600E (T>A), S607S (TCC>AGT)
    Promoter
    None for primary insert. PGK for puromycin resist…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183078-plasmid-map-sequence-id-363420

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  35. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  36. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

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  37. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  38. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  39. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

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  40. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    131730-plasmid-map-sequence-id-318177

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