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pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM62
Plasmid#154160PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel2-specific…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEThT-MAHS delta MTP
Plasmid#90032PurposeTo express His-tagged MAHS in bacteriaDepositorInsertMAHS
UseTagsHisExpressionBacterialMutationDeletion of mitochondria targeting peptidePromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-Zyxin-6
Plasmid#55101PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertZyxin (ZYX Human)
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorInsertCasPhi-2
UseTags2xSV40NLS-2xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-ZIBG2
Plasmid#169458Purposecell surface localized zinc sensor with ~ 300 nM KdDepositorInsertZIBG2
UseTagspDisplay backbone elements for export and membran…ExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CalfluxCTN
Plasmid#190068PurposeExpresses BRET Ca2+ sensor CalfluxCTN in mammalian cellsDepositorInsertCalfluxCTN
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pKDC.015
Plasmid#184992PurposeExpress -Eco2 RT and ncRNA in human cellsDepositorInsertEco2: RT and ncRNA(wt), a1/a2 length: 13
UseTagsExpressionMammalianMutationHuman codon optimized RTPromoterTetOn-3gAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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CMV-Y-GECO2m
Plasmid#105070PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cellsDepositorInsertY-GECO2m
UseTags6x His tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.2s
Plasmid#105069PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
H5_fold-0 _Chantal
Plasmid#201826PurposeExpresses H5_fold-0_Chantal in bacterial cellsDepositorInsertH5_fold-0 _Chantal
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2m
Plasmid#105066PurposeA yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cellsDepositorInsertY-GECO2m
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2s
Plasmid#105067PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2.1s
Plasmid#105068PurposeA yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cellsDepositorInsertY-GECO2.1s
UseTags6x His tagExpressionBacterialMutationPromoterpBADAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(L53P,L67P)
Plasmid#172458PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutantDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationchanged Leucines 53 and 67 to proline (breaks coi…PromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH1-FLAG
Plasmid#66802PurposeTet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only