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pcDNA3.1(+) eGFP PPP1R8 G4

Plasmid

#129021

Purpose

Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP EML2 G4

Plasmid

#129023

Purpose

Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-SUN1 EGFP

Plasmid

#187675

Purpose

In vivo visualization of SUN1

Depositor

Mary Beckerle

Insert

SUN1 (Sun1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Cplx2-GFP KI

Plasmid

#131476

Purpose

Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cplx2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR6k-con-GFPuv

Plasmid

#106387

Purpose

Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

LlacO-1

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-mGFP-pA(AV222)

Plasmid

#117858

Purpose

This plasmid expresses membrane anchored GFP to facility axon morphology study.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

mGFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-GFP

Plasmid

#105946

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC (Prkcz Rat)

Use

Tags

CAAX and GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-G380R

Plasmid

#191755

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Use

Tags

mGFP

Expression

Mammalian

Mutation

c.1138G>A (G380R) + c.1131C>T (silent mutat…

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPlacPbuCas13b-gRNA-T-deGFP

Plasmid

#184840

Purpose

Expression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

single-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119 and lac promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-GFP

Plasmid

#178712

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCg-C1

Plasmid

#112266

Purpose

ITRACKgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-851 (Vh)

Plasmid

#46268

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Use

Tags

msGFPx3

Expression

Yeast

Mutation

Promoter

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-KD

Plasmid

#25929

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

RapR-FAK (Ptk2 Human, Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-KD plasmid contains the FA…

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX NM_178152.2, Human)

Use

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-UBC:GFP

Plasmid

#68482

Purpose

Retrovirus expressing GFP driven by UBC promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR and UBC

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBEAST-pBen-sfGFP

Plasmid

#114598

Purpose

"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Tags

Expression

Mutation

Promoter

PBen

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.GFP-pcDNA3

Plasmid

#204360

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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