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pHH0103_YWHAH_14-3-3

Plasmid

#109988

Purpose

Protein expression and purification of YWHAH_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAH_14-3-3 (YWHAH Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RPA1_RepA-N

Plasmid

#110032

Purpose

Protein expression and purification of RPA1_RepA-N

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RPA1_RepA-N (RPA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SIAH2_Sina

Plasmid

#109813

Purpose

Protein expression and purification of SIAH2_Sina

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SIAH2_Sina (SIAH2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A3701

Plasmid

#91052

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-dAtCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-PDZ2

Plasmid

#28296

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains aa 148-298 (deletion of the first PDZ do…

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pKV016

Plasmid

#64767

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSC201

Plasmid

#129387

Purpose

A variant of the pSCrhaB2 vector with the oriR6K and mob genes from pGPΩ-TP. Used for homologous recombination of the rhamnose-inducible system into the chromosome of Burkholderia cenocepacia K56-2.

Depositor

Silvia Cardona

Article

Ortega et al J Bacteriol. 2007 May;189(9):3639-44. do

Insert

oriR6K and oriT

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

N/A

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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bactin:GFP-DCX

Plasmid

#105961

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKM263-EcPPXc

Plasmid

#38328

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Use

Tags

6xHIS, GST, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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JPF0510

Plasmid

#124001

Purpose

Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Tags

Expression

Yeast

Mutation

Promoter

PADH1

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Use

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pSR33

Plasmid

#69254

Purpose

phsp16.2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hsp16.2

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MTK2_004

Plasmid

#123699

Purpose

Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAtipiTrc

Plasmid

#53275

Purpose

Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

Insert

ipi1 (IPP2 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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