-
Plasmid#175771PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639DDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutation632-639DPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN D474N
Plasmid#175762PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN D474NDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationD474NPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKH115_GALNT2-keima
Plasmid#214969PurposeGolgiphagy flux reporter GALNT2-keimaDepositorInsertGALNT2-keima (GALNT2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART Tmem192-3X HA (targeting vector for genomic tagging)
Plasmid#175777PurposepSMART Tmem192-3X HA (targeting vector for genomic tagging)DepositorInsertTMEM192 (TMEM192 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-NDP52
Plasmid#175749PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKH162_FLAG-APEX2-YIPF3 LIR mut
Plasmid#214981PurposeFlag-APEX2 tagged LIR mutant YIPF3DepositorInsertFLAG-APEX2-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS1
Plasmid#175755PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1DepositorInsertLGALS1 (LGALS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9-HE-Geminin
Plasmid#109402PurposeExpresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degronDepositorInsertHE-Geminin
UseTagsExpressionMutationPromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-CALCOCO2
Plasmid#175759PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-CALCOCO2/NDP52DepositorInsertNDP52/CALCOCO2 (CALCOCO2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS8
Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN S473A 513A
Plasmid#175763PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN S473A 513ADepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationS473A S513APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-EEA1
Plasmid#176491PurposeFor lentiviral transduction of 3FLAG-EEA1DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mKeima-LGALS3
Plasmid#175780PurposeLentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic fluxDepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23Available sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKH151_PB_flag-LC3B_wt_IRES-BFP
Plasmid#214976PurposeFlag tagged LC3BDepositorInsertFLAG-LC3B (MAP1LC3B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only