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Plasmid#201479PurposeBRET detection of V5-tagged proteinDepositorInsertNbV5-Rluc8
UseTagsRluc8ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGas-miRFP670nano3
Plasmid#184672PurposeG protein alpha-subunit internally tagged with miRFP670nano3DepositorInsertG protein alpha-s-miRFP670nano3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3LCA_TM287/288
Plasmid#132701Purposeexpresses TM287/288 in E.coli, to use as positive control in ELISADepositorUseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVIM-BC2T
Plasmid#108967Purposeexpresses vimentin-BC2-tag in mammalian cellsDepositorInsertVimentin
UseTagsBC2TExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynSNAP IRES EGFP
Plasmid#136625PurposePlasmid encoding outer inner membrane localization of SNAP-Tag and EGFPDepositorInsertLyn11-SNAP-Tag (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASp-GBP-Tbro
Plasmid#179995PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-yibDp-Erv1p
Plasmid#202483PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorInsertsulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p
Plasmid#202484PurposeExpresses sulfhydryl oxidase (Erv1p) constitutivelyDepositorInsertsulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterEM7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-roGFP2
Plasmid#202462PurposeExpresses redox sensitive GFP (roGFP) after phosphate depletionDepositorInsertreduction-oxidation sensitive green fluorescent protein
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-roGFP
Plasmid#202464PurposeExpresses redox sensitive GFP (roGFP) with T7 inductionDepositorInsertreduction-oxidation sensitive green fluorescent protein
UseTags6X-HisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-yibDp-Erv1p
Plasmid#212094PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorInsertSulfydryl oxidase (ERV1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
NbV5-TEV219S153N
Plasmid#201488PurposeTANGO detection of V5-tagged proteinDepositorInsertNbV5-TEV219S153N
UseTagsTEV219 S153NExpressionMammalianMutationS153N in TEV219PromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-miRFP670nano3
Plasmid#184674PurposeSignal anchor of rabbit cytochrome p450 and miRFP670nano3DepositorInsertCytERM-miRFP670nano3
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_C1
Plasmid#171924PurposeBacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C1
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPINO_C5
Plasmid#171925PurposeBacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C5
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS
Plasmid#199351PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFAExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP
Plasmid#199363PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-Lag16-iCAM-1
Plasmid#205195PurposeThis vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP
Plasmid#199359PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only