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Addgene
Showing: 521 - 539 of 539 results

  1. Nabi2.216

    Plasmid
    #73801
    Purpose
    Nabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.216
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    73801-plasmid-map-sequence-id-136840

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  2. Nabi2.226

    Plasmid
    #73802
    Purpose
    Nabi2.226 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.
    Depositor
    Bradley Baker
    Article
    Sung et al PLoS One. 2015 Nov 20;10(11):e0141585. d
    Insert
    Nabi2.226
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    73802-plasmid-map-sequence-id-136841

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  9. MTK0_011

    Plasmid
    #123966
    Purpose
    Encodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hRosa26-PhiC31 landing pad
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123966-plasmid-map-sequence-id-251672

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  10. MTK0_012

    Plasmid
    #123967
    Purpose
    Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    hRosa26-BxBI landing pad
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    123967-plasmid-map-sequence-id-251720

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  12. pKL2-138

    Plasmid
    #219413
    Purpose
    Estradiol-inducible expression of 42-10 Zinc finger activator
    Depositor
    Ahmad Khalil
    Article
    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.
    Insert
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
    Use
    Tags
    Expression
    Yeast
    Mutation
    Four Arg to Ala mutations in zinc finger backbone
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    219413-plasmid-map-sequence-id-434474

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  13. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  15. pYSD313

    Plasmid
    #212931
    Purpose
    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Article
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    Sed1 (SED1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    212931-plasmid-map-sequence-id-421768

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  19. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Article
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    122242-plasmid-map-sequence-id-238909

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Showing: 521 - 539 of 539 results