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pLNL1141

Plasmid

#160867

Purpose

PJ23110-RBS-PhCFAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Coniferyl alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Mutation

Promoter

PJ23110

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1127

Plasmid

#160859

Purpose

PJ23110-RBS-RhAAT1-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Acetyl CoA geraniol/citronellol acetyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Mutation

Promoter

PJ23110

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0626

Plasmid

#129001

Purpose

pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0627

Plasmid

#129002

Purpose

pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0625

Plasmid

#129000

Purpose

pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_031

Plasmid

#123970

Purpose

Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_030

Plasmid

#123969

Purpose

Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phiC31-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ARB343

Plasmid

#124026

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::mRuby2-bgPA_R1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB351

Plasmid

#124034

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mRuby2

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::mRuby2-SV40_RE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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ARB347

Plasmid

#124030

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::mRuby2-rglPA_R2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_010

Plasmid

#123823

Purpose

Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

T2A::NLS::mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_021

Plasmid

#123834

Purpose

Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

T2A::NLS::mRuby2::PEST4d

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_032

Plasmid

#123746

Purpose

Encodes JNK Translocation Reporter (JNK KTR) fused to mRuby2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

JNKKTR-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B2

Plasmid

#172843

Purpose

CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mRuby3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMM0638

Plasmid

#129005

Purpose

Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0641

Plasmid

#129006

Purpose

Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYSD153

Plasmid

#212929

Purpose

Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYSD141

Plasmid

#212928

Purpose

Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge